8E9C

Crystal structure of E. coli aspartate aminotransferase mutant AIFS in the ligand-free form at 100 K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Aspartate aminotransferase	polymer	406	44751.4	2	UniProt (P00509) Pfam (PF00155) In PDB	Escherichia coli	AspAT,Transaminase A
2	A, B	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
3	A, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4		water	water		18.0	172	Chemie (HOH)

Sequence modifications

A, B: 2 - 396 (UniProt: P00509)

PDB	External Database	Details
Met -9	-	initiating methionine
Ala -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Val -1	-	expression tag
Gly 0	-	expression tag
Thr 1	-	expression tag
Ala 35	Val 35	engineered mutation
Ile 37	Lys 37	engineered mutation
Phe 43	Thr 43	engineered mutation
Ser 64	Asn 64	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	89502.8
Non-Polymers*	Number of molecules	4
	Total formula weight	686.4
All*	Total formula weight	90189.3

*Water molecules are not included.