8E8Q
Cryo-EM structure of substrate-free DNClpX.ClpP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... | ATP-dependent Clp protease ATP-binding subunit ClpX | polymer | 388 | 42355.8 | 6 | UniProt (P0A6H1) Pfam (PF07724) Pfam (PF10431) In PDB | Escherichia coli | ATP-dependent unfoldase ClpX |
| 2 | H, I, J, K, L... | ATP-dependent Clp protease proteolytic subunit | polymer | 207 | 23468.9 | 14 | UniProt (P0A6G7) Pfam (PF00574) In PDB | Escherichia coli | Caseinolytic protease,Endopeptidase Clp,Heat shock protein F21.5,Protease Ti |
| 3 | A, B, C, D | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 4 | Chemie (ATP) | |||
| 4 | D, B, C | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 5 | E, F | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) |
Sequence modifications
A, B, C, D, E, F: 62 - 424 (UniProt: P0A6H1)
H, I, J, K, L, M, N, h, i, j, k, l, m, n: 2 - 193 (UniProt: P0A6G7)
| PDB | External Database | Details |
|---|---|---|
| Met 37 | - | expression tag |
| Gly 38 | - | expression tag |
| Ser 39 | - | expression tag |
| Ser 40 | - | expression tag |
| His 41 | - | expression tag |
| His 42 | - | expression tag |
| His 43 | - | expression tag |
| His 44 | - | expression tag |
| His 45 | - | expression tag |
| His 46 | - | expression tag |
| Asp 47 | - | expression tag |
| Tyr 48 | - | expression tag |
| Asp 49 | - | expression tag |
| Ile 50 | - | expression tag |
| Pro 51 | - | expression tag |
| Thr 52 | - | expression tag |
| Thr 53 | - | expression tag |
| Glu 54 | - | expression tag |
| Asn 55 | - | expression tag |
| Leu 56 | - | expression tag |
| Tyr 57 | - | expression tag |
| Phe 58 | - | expression tag |
| Gln 59 | - | expression tag |
| Gly 60 | - | expression tag |
| Ser 61 | - | expression tag |
| Ser 169 | Cys 169 | engineered mutation |
| Glu 408 | Lys 408 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Glu 194 | - | expression tag |
| Asn 195 | - | expression tag |
| Leu 196 | - | expression tag |
| Tyr 197 | - | expression tag |
| Phe 198 | - | expression tag |
| Gln 199 | - | expression tag |
| Ser 200 | - | expression tag |
| Leu 201 | - | expression tag |
| Glu 202 | - | expression tag |
| His 203 | - | expression tag |
| His 204 | - | expression tag |
| His 205 | - | expression tag |
| His 206 | - | expression tag |
| His 207 | - | expression tag |
| His 208 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 20 |
| Total formula weight | 582699.0 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 2956.0 | |
| All* | Total formula weight | 585655.1 |
