8E5E
Crystal structure of double-stranded DNA deaminase toxin DddA in complex with DNA with the target cytosine flipped into the active site
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Double-stranded DNA deaminase toxin A | polymer | 139 | 14686.2 | 1 | UniProt (P0DUH5) Pfam (PF14428) In PDB | Burkholderia cenocepacia | DddA,Cytidine deaminase,CD |
2 | C | DNA (5'-D(*GP*TP*AP*CP*CP*GP*GP*AP*CP*GP*TP*TP*GP*C)-3') | polymer | 14 | 4296.8 | 1 | synthetic construct | ||
3 | B | DNA (5'-D(*GP*CP*AP*AP*CP*GP*TP*CP*CP*GP*GP*TP*AP*C)-3') | polymer | 14 | 4265.8 | 1 | synthetic construct | ||
4 | A | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
5 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
6 | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence modifications
A: 1290 - 1422 (UniProt: P0DUH5)
PDB | External Database | Details |
---|---|---|
Ala 1347 | Glu 1347 | engineered mutation |
His 1423 | - | expression tag |
His 1424 | - | expression tag |
His 1425 | - | expression tag |
His 1426 | - | expression tag |
His 1427 | - | expression tag |
His 1428 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 23248.8 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 89.7 | |
All* | Total formula weight | 23338.5 |