8DWE
Adenine glycosylase MutY variant E43Q in complex with DNA containing d(8-oxo-G) paired with substrate purine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | Adenine DNA glycosylase | polymer | 365 | 41772.6 | 2 | UniProt (P83847) Pfam (PF00730) Pfam (PF00633) Pfam (PF10576) Pfam (PF14815) | Geobacillus stearothermophilus | oxoG:A-specific adenine glycosylase |
| 2 | B, E (B, E) | DNA (5'-D(*AP*AP*GP*AP*CP*(8OG)P*TP*GP*GP*AP*C)-3') | polymer | 11 | 3423.2 | 2 | synthetic construct | ||
| 3 | C, F (C, F) | DNA (5'-D(*TP*GP*TP*CP*CP*AP*(PRN)P*GP*TP*CP*T)-3') | polymer | 11 | 3309.2 | 2 | synthetic construct | ||
| 4 | G, L (A, D) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
| 5 | H, M (A, D) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 6 | I, J, N, O (A, D) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 7 | K, P (B, E) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 2 | Chemie (TRS) | |||
| 8 | Q, R, S, T, U... (A, B, C, D, E...) | water | water | 18.0 | 204 | Chemie (HOH) |
Sequence modifications
A, D: 1 - 365 (UniProt: P83847)
| PDB | External Database | Details |
|---|---|---|
| Gln 43 | Glu 43 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 97010.1 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1232.0 | |
| All* | Total formula weight | 98242.1 |
