8DQJ
Crystal structure of pyrrolysyl-tRNA synthetase from Methanomethylophilus alvus engineered for acridone amino acid (AST) bound to ATP and acridone
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (D, A) | AA_TRNA_LIGASE_II domain-containing protein | polymer | 276 | 30750.9 | 2 | UniProt (A0A3G3IHP7) Pfam (PF17759) | Candidatus Methanomethylophilus alvus | |
| 2 | C (D) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 3 | D (D) | (2~{S})-2-azanyl-3-(9-oxidanylidene-10~{H}-acridin-2-yl)propanoic acid | non-polymer | 283.3 | 1 | Chemie (T7Q) | |||
| 4 | E, F, G (D) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 5 | H (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | I (A) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) | |||
| 7 | J (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 8 | K, L (D, A) | water | water | 18.0 | 374 | Chemie (HOH) |
Sequence modifications
D, A: 2 - 275 (UniProt: A0A3G3IHP7)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| Ser 166 | Asn 166 | engineered mutation |
| Cys 168 | Val 168 | engineered mutation |
| Thr 239 | Trp 239 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 61501.8 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1408.8 | |
| All* | Total formula weight | 62910.7 |
