8DQJ
Crystal structure of pyrrolysyl-tRNA synthetase from Methanomethylophilus alvus engineered for acridone amino acid (AST) bound to ATP and acridone
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | D, A | AA_TRNA_LIGASE_II domain-containing protein | polymer | 276 | 30750.9 | 2 | UniProt (A0A3G3IHP7) Pfam (PF17759) In PDB | Candidatus Methanomethylophilus alvus | |
2 | D | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
3 | D | (2~{S})-2-azanyl-3-(9-oxidanylidene-10~{H}-acridin-2-yl)propanoic acid | non-polymer | 283.3 | 1 | Chemie (T7Q) | |||
4 | D | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
5 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
6 | A | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) | |||
7 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
8 | water | water | 18.0 | 374 | Chemie (HOH) |
Sequence modifications
D, A: 2 - 275 (UniProt: A0A3G3IHP7)
PDB | External Database | Details |
---|---|---|
Ser 0 | - | expression tag |
Gly 1 | - | expression tag |
Ser 166 | Asn 166 | engineered mutation |
Cys 168 | Val 168 | engineered mutation |
Thr 239 | Trp 239 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 61501.8 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 1408.8 | |
All* | Total formula weight | 62910.7 |