8DQG
Crystal structure of pyrrolysyl-tRNA synthetase from Methanomethylophilus alvus engineered for acridone amino acid (RS1) bound to AMPPNP and acridone
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | D, A | AA_TRNA_LIGASE_II domain-containing protein | polymer | 276 | 30690.9 | 2 | UniProt (A0A3G3IHP7) Pfam (PF17759) In PDB | Candidatus Methanomethylophilus alvus | |
| 2 | D, A | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 2 | Chemie (ANP) | |||
| 3 | D | (2~{S})-2-azanyl-3-(9-oxidanylidene-10~{H}-acridin-2-yl)propanoic acid | non-polymer | 283.3 | 1 | Chemie (T7Q) | |||
| 4 | D, A | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 5 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 7 | water | water | 18.0 | 509 | Chemie (HOH) |
Sequence modifications
D, A: 2 - 275 (UniProt: A0A3G3IHP7)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| Ala 166 | Asn 166 | engineered mutation |
| Gly 168 | Val 168 | engineered mutation |
| Cys 239 | Trp 239 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 61381.7 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1615.4 | |
| All* | Total formula weight | 62997.2 |
