8DPI

Cryo-EM structure of the 5HT2C receptor (VSV isoform) bound to lorcaserin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	5-hydroxytryptamine receptor 2C	polymer	458	51821.0	1	UniProt (P28335) Pfam (PF00001) In PDB	Homo sapiens (human)	5-HT-2C,5-HT2C,5-HTR2C,5-hydroxytryptamine receptor 1C,5-HT-1C,5-HT1C,Serotonin receptor 2C
2	B	G-alpha subunit q (Gi2-mini-Gq chimera)	polymer	246	28084.8	1		Homo sapiens
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
4	D	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
5	E	Antibody fragment scFv16	polymer	267	28668.9	1		Homo sapiens
6	A	(1R)-8-chloro-1-methyl-2,3,4,5-tetrahydro-1H-3-benzazepine	non-polymer		195.7	1	Chemie (T4U)
7	A	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)

Sequence modifications

A: 1 - 458 (UniProt: P28335)

PDB	External Database	Details
Cys 23	Ser 23	variant
Val 156	Ile 156	variant
Ser 158	Asn 158	variant
Val 160	Ile 160	variant

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	155854.0
Non-Polymers*	Number of molecules	2
	Total formula weight	582.3
All*	Total formula weight	156436.3

*Water molecules are not included.