8DPG

Cryo-EM structure of the 5HT2C receptor (INI isoform) bound to psilocin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	5-hydroxytryptamine receptor 2C	polymer	458	51876.1	1	UniProt (P28335) Pfam (PF00001)	Homo sapiens (human)	5-HT-2C,5-HT2C,5-HTR2C,5-hydroxytryptamine receptor 1C,5-HT-1C,5-HT1C,Serotonin receptor 2C
2	B (B)	G-alpha subunit q (Gi2-mini-Gq chimera)	polymer	246	28084.8	1		Homo sapiens
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
4	D (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (E)	Antibody fragment scFv16	polymer	267	28668.9	1		Homo sapiens
6	F (A)	3-[2-(dimethylamino)ethyl]-1~{H}-indol-4-ol	non-polymer		204.3	1	Chemie (91Q)
7	G (A)	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)

Sequence modifications

A: 1 - 458 (UniProt: P28335)

PDB	External Database	Details
Cys 23	Ser 23	variant

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	155909.0
Non-Polymers*	Number of molecules	2
	Total formula weight	590.9
All*	Total formula weight	156500.0

*Water molecules are not included.