8DPD

superfolder GFP Tyr74pCNPhe mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Superfolder green fluorescent protein	polymer	239	26907.3	1	UniProt (P42212) Pfam (PF01353)	Aequorea victoria
2	B (A)	CARBON DIOXIDE	non-polymer		44.0	1	Chemie (CO2)
3	C, D, E, F (A)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
4	G (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	H, I, J, K (A)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
6	L (A)	water	water		18.0	227	Chemie (HOH)

Sequence modifications

A: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Met 0	-	initiating methionine
Val 1	-	expression tag
Arg 30	Ser 30	engineered mutation
Asn 39	Tyr 39	engineered mutation
Leu 64	Phe 64	engineered mutation
Cro 66	Ser 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
4cf 74	Tyr 74	engineered mutation
Arg 80	Gln 80	engineered mutation
Ser 99	Phe 99	engineered mutation
Thr 105	Asn 105	engineered mutation
Phe 145	Tyr 145	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Val 171	Ile 171	engineered mutation
Val 206	Ala 206	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	26907.3
Non-Polymers*	Number of molecules	10
	Total formula weight	495.6
All*	Total formula weight	27403.0

*Water molecules are not included.