8DOQ

Crystal structure of 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatase from Klebsiella aerogenes (P21 Form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatase	polymer	253	27335.4	2	UniProt (A0A0M3H420) In PDB	Klebsiella aerogenes	Orotidine 5'-phosphate decarboxylase,OMP decarboxylase,OMPDCase,OMPdecase
2	A	BENZOIC ACID	non-polymer		122.1	1	Chemie (BEZ)
3	A, B	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	2	Chemie (MPD)
4		water	water		18.0	307	Chemie (HOH)

Sequence modifications

A, B: 9 - 249 (UniProt: A0A0M3H420)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
Glu 42	Asp 34	engineered mutation
Met 152	Thr 144	engineered mutation
Val 182	Ala 174	engineered mutation
Val 239	Ala 231	engineered mutation
Asn 250	-	expression tag
Lys 251	-	expression tag
Gly 252	-	expression tag
Val 253	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	54670.9
Non-Polymers*	Number of molecules	3
	Total formula weight	358.5
All*	Total formula weight	55029.3

*Water molecules are not included.