8DOP

Crystal structure of 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatase from Klebsiella aerogenes (P1 Form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatase	polymer	253	27335.4	8	UniProt (A0A0M3H420)	Klebsiella aerogenes (Enterobacter aerogenes)	Orotidine 5'-phosphate decarboxylase,OMP decarboxylase,OMPDCase,OMPdecase
2	I, J, L, N, O... (A, B, C, D, E...)	IODIDE ION	non-polymer		126.9	12	Chemie (IOD)
3	K, M, P, R, U... (A, B, C, D, E...)	SODIUM ION	non-polymer		23.0	6	Chemie (NA)
4	AA, BA, CA, DA, EA... (A, B, C, D, E...)	water	water		18.0	390	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: -10 - 230 (UniProt: A0A0M3H420)

PDB	External Database	Details
Met -18	-	initiating methionine
Ala -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Glu 23	Asp 34	engineered mutation
Met 133	Thr 144	engineered mutation
Val 163	Ala 174	engineered mutation
Val 220	Ala 231	engineered mutation
Asn 231	-	expression tag
Lys 232	-	expression tag
Gly 233	-	expression tag
Val 234	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	218683.5
Non-Polymers*	Number of molecules	18
	Total formula weight	1660.8
All*	Total formula weight	220344.3

*Water molecules are not included.