8DOK
Cryo-EM structure of T/F100 SOSIP.664 HIV-1 Env trimer in complex with 8ANC195 and 10-1074
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E, I (A, E, I) | CRF-1_AE T/F100 Env gp120 | polymer | 486 | 54739.1 | 3 | UniProt (A0A140EMT3) Pfam (PF00516) | Human immunodeficiency virus 1 | |
| 2 | B, F, J (B, F, J) | CRF-1_AE T/F100 HIV-1 gp41 | polymer | 155 | 17389.8 | 3 | UniProt (A0A6C0ZY47) Pfam (PF00517) | Human immunodeficiency virus 1 | Env polyprotein |
| 3 | C, G, K (C, G, K) | Heavy chain of 8ANC195 | polymer | 238 | 25324.4 | 3 | Homo sapiens (Human) | ||
| 4 | D, H, L (D, H, L) | Light chain of 8ANC195 | polymer | 215 | 23460.0 | 3 | Homo sapiens (Human) | ||
| 5 | M, O, Q (M, O, Q) | Heavy chain of 10-1074 | polymer | 238 | 25661.7 | 3 | Homo sapiens (Human) | ||
| 6 | N, P, R (N, P, R) | Light chain of 10-1074 | polymer | 214 | 23180.8 | 3 | Homo sapiens (Human) | ||
| 7 | DA, S (i, S) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1559.4 | 2 | In PDB GlyTouCan (G04414GO) | |||
| 8 | BA, CA, JA, KA, T... (g, h, r, s, T...) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 7 | In PDB GlyTouCan (G15407YE) | |||
| 9 | AA, GA, U (b, m, U) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 830.8 | 3 | In PDB GlyTouCan (G01361SX) | |||
| 10 | FA, HA, IA, V (l, p, q, V) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 4 | In PDB GlyTouCan (G42666HT) | |||
| 11 | Y (Y) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 1 | In PDB GlyTouCan (G40702WU) | |||
| 12 | Z (Z) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G56014GC) | |||
| 13 | EA (j) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 14 | AB, AC, BB, CB, DB... (E, K, F, A, G...) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 42 | Chemie (NAG) |
Sequence modifications
A, E, I: 30 - 507 (UniProt: A0A140EMT3)
B, F, J: 512 - 664 (UniProt: A0A6C0ZY47)
| PDB | External Database | Details |
|---|---|---|
| Cys 501 | Ala 502 | conflict |
| Arg 508 | - | expression tag |
| Arg 509 | - | expression tag |
| Arg 510 | - | expression tag |
| Arg 511 | - | expression tag |
| Arg 512 | - | expression tag |
| Arg 513 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 560 | conflict |
| Cys 605 | Thr 606 | conflict |
| Ala 665 | - | expression tag |
| Ala 666 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 18 |
| Total formula weight | 509267.4 | |
| Branched | Number of molecules | 19 |
| Total formula weight | 15119.8 | |
| Non-Polymers* | Number of molecules | 42 |
| Total formula weight | 9290.7 | |
| All* | Total formula weight | 533678.0 |
