8DNX

Cryo-EM structure of the human Sec61 complex inhibited by cotransin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Protein transport protein Sec61 subunit gamma	polymer	68	7752.3	1	UniProt (P60059) Pfam (PF00584)	Homo sapiens (human)
2	B (C)	Protein transport protein Sec61 subunit beta	polymer	96	9987.5	1	UniProt (P60468) Pfam (PF03911)	Homo sapiens (human)
3	C (A)	Protein transport protein Sec61 subunit alpha isoform 1	polymer	476	52202.4	1	UniProt (P61619) Pfam (PF10559) Pfam (PF00344)	Homo sapiens (human)	Sec61 alpha-1
4	D (D)	Cotransin analogue peptide inhibitor	polymer	7	953.2	1		synthetic construct

Sequence modifications

A: 1 - 476 (UniProt: P61619)

PDB	External Database	Details
Tyr 263	Val 263	conflict
Glu 264	Asp 264	engineered mutation
Arg 268	Lys 268	engineered mutation
Thr 270	Ala 270	engineered mutation
Lys 271	Arg 271	engineered mutation
Val 272	Tyr 272	engineered mutation
Ile 276	Tyr 276	engineered mutation
Gly 277	Asn 277	engineered mutation
Ile 278	Thr 278	engineered mutation
Pro 387	Ala 387	conflict
Arg 388	Lys 388	conflict
Ile 390	Val 390	conflict
Phe 394	Leu 394	engineered mutation
Asp 396	Glu 396	conflict
Gly 398	Gln 398	conflict
Ile 401	Met 401	engineered mutation
Asn 402	Arg 402	engineered mutation
Lys 404	His 404	engineered mutation
Ile 409	Met 409	engineered mutation
Tyr 410	Val 410	engineered mutation
Arg 411	His 411	engineered mutation
Lys 414	Asn 414	conflict
Lys 415	Arg 415	conflict
Ile 416	Tyr 416	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	70895.4
All*	Total formula weight	70895.4

*Water molecules are not included.