8DN3

Cryo-EM structure of human Glycine Receptor alpha1-beta heteromer, apo state

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (D, A, B, C)	Glycine receptor subunit alpha-1	polymer	367	42421.1	4	UniProt (P23415) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	Glycine receptor 48 kDa subunit,Glycine receptor strychnine-binding subunit
2	E (E)	Glycine receptor subunit beta,Green fluorescent protein,Glycine receptor beta	polymer	680	76584.5	1	UniProt (P48167) UniProt (P42212) UniProt (A0A2K6CAQ3) Pfam (PF02931) Pfam (PF02932) Pfam (PF01353)	Homo sapiens (human)
3	EA, EB, F, QA, S (B, E, D, C, A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	5	Chemie (NAG)
4	BA, CB, DA, FA, G... (A, E, B, D, C)	HEXANE	non-polymer		86.2	15	Chemie (HEX)
5	AA, GB, H, I, LB... (A, E, D, C)	UNDECANE	non-polymer		156.3	12	Chemie (UND)
6	AB, CA, HA, HB, IA... (C, A, B, E, D)	nonane	non-polymer		128.3	13	Chemie (DD9)
7	FB, K, KB, LA, O (E, D, B)	N-BUTANE	non-polymer		58.1	5	Chemie (NBU)
8	BB, DB, M, N, PA... (E, D, C, A)	HEPTANE	non-polymer		100.2	8	Chemie (HP6)
9	UA (C)	DECANE	non-polymer		142.3	1	Chemie (D10)
10	MB, NB (E)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)

Sequence modifications

D, A, B, C: 1 - 428 (UniProt: P23415)

PDB	External Database	Details
-	Arg 344	deletion
-	Phe 345	deletion
-	Arg 346	deletion
-	Arg 347	deletion
-	Lys 348	deletion
-	Arg 349	deletion
-	Arg 350	deletion
-	His 351	deletion
-	His 352	deletion
-	Lys 353	deletion
-	Ser 354	deletion
-	Pro 355	deletion
-	Met 356	deletion
-	Leu 357	deletion
-	Asn 358	deletion
-	Leu 359	deletion
-	Phe 360	deletion
-	Gln 361	deletion
-	Glu 362	deletion
-	Asp 363	deletion
-	Glu 364	deletion
-	Ala 365	deletion
-	Gly 366	deletion
-	Glu 367	deletion
-	Gly 368	deletion
-	Arg 369	deletion
-	Phe 370	deletion
-	Asn 371	deletion
-	Phe 372	deletion
-	Ser 373	deletion
-	Ala 374	deletion
-	Tyr 375	deletion
-	Gly 376	deletion
-	Met 377	deletion
-	Gly 378	deletion
-	Pro 379	deletion
-	Ala 380	deletion
-	Cys 381	deletion
-	Leu 382	deletion
-	Gln 383	deletion
-	Ala 384	deletion
-	Lys 385	deletion
-	Asp 386	deletion
-	Gly 387	deletion
-	Ile 388	deletion
-	Ser 389	deletion
-	Val 390	deletion
-	Lys 391	deletion
-	Gly 392	deletion
-	Ala 393	deletion
-	Asn 394	deletion
-	Asn 395	deletion
-	Ser 396	deletion
-	Asn 397	deletion
-	Thr 398	deletion
-	Thr 399	deletion
-	Asn 400	deletion
-	Pro 401	deletion
-	Pro 402	deletion
-	Pro 403	deletion
-	Ala 404	deletion
-	Pro 405	deletion
Gly 377	Ser 406	conflict
Ser 378	Lys 407	conflict
Gly 380	Pro 409	conflict

E: 3 - 333 (UniProt: P48167)

PDB	External Database	Details
Gly 333	-	linker
Gly 333	-	linker
Ser 333	-	linker
Ser 333	-	linker
Ala 333	-	linker
Ala 333	-	linker
Ala 333	-	linker

E: 333 - 342 (UniProt: P42212)

PDB	External Database	Details
Val 333	Met 1	conflict
Ser 342	-	linker
Gly 342	-	linker

E: 342 - 475 (UniProt: A0A2K6CAQ3)

PDB	External Database	Details
Gly 342	Thr 380	conflict
Ser 342	Leu 381	conflict
Gly 342	Gln 382	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	246269.0
Non-Polymers*	Number of molecules	61
	Total formula weight	7247.1
All*	Total formula weight	253516.1

*Water molecules are not included.