8DMI

Lymphocytic choriomeningitis virus glycoprotein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, B, C)	Glycoprotein G1	polymer	225	25521.2	3	UniProt (P09991) Pfam (PF00798)	Lymphocytic choriomeningitis virus
2	B, D, F (a, b, c)	Glycoprotein G2,Cobalamin adenosyltransferase-like domain-containing protein trimerization tag	polymer	375	42166.8	3	UniProt (P09991) UniProt (Q9HIA7) Pfam (PF00798) Pfam (PF01923)	Lymphocytic choriomeningitis virus	GP2
3	G, H, I, J, K... (D, E, F, G, H...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	18	In PDB GlyTouCan (G42666HT)
4	AA, BA, CA, DA, Y... (B, b, C, c, A...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A, B, C: 58 - 265 (UniProt: P09991)

PDB	External Database	Details
Met 41	-	initiating methionine
Lys 42	-	expression tag
Leu 43	-	expression tag
Cys 44	-	expression tag
Ile 45	-	expression tag
Leu 46	-	expression tag
Leu 47	-	expression tag
Ala 48	-	expression tag
Val 49	-	expression tag
Val 50	-	expression tag
Ala 51	-	expression tag
Phe 52	-	expression tag
Val 53	-	expression tag
Gly 54	-	expression tag
Leu 55	-	expression tag
Ser 56	-	expression tag
Leu 57	-	expression tag
Cys 207	Gly 207	engineered mutation
Leu 260	Phe 260	conflict
Arg 264	Leu 264	engineered mutation
Arg 265	Ala 265	engineered mutation

a, b, c: 266 - 430 (UniProt: P09991)

PDB	External Database	Details
Glu 313	Ala 313	variant
Pro 334	Glu 334	engineered mutation
Cys 366	Gly 366	engineered mutation
Ala 398	Ser 398	engineered mutation
Leu 431	-	linker
Glu 432	-	linker
Gly 433	-	linker
Gly 434	-	linker

a, b, c: 435 - 583 (UniProt: Q9HIA7)

PDB	External Database	Details
Val 522	Ile 110	conflict
Ile 571	Leu 159	conflict
Ala 584	-	expression tag
Leu 585	-	expression tag
Glu 586	-	expression tag
Val 587	-	expression tag
Asp 588	-	expression tag
Asp 589	-	expression tag
Asp 590	-	expression tag
Asp 591	-	expression tag
Lys 592	-	expression tag
Ala 593	-	expression tag
Gly 594	-	expression tag
Trp 595	-	expression tag
Ser 596	-	expression tag
His 597	-	expression tag
Pro 598	-	expression tag
Gln 599	-	expression tag
Phe 600	-	expression tag
Glu 601	-	expression tag
Lys 602	-	expression tag
Gly 603	-	expression tag
Gly 604	-	expression tag
Gly 605	-	expression tag
Ser 606	-	expression tag
Gly 607	-	expression tag
Gly 608	-	expression tag
Gly 609	-	expression tag
Ser 610	-	expression tag
Gly 611	-	expression tag
Gly 612	-	expression tag
Gly 613	-	expression tag
Ser 614	-	expression tag
Trp 615	-	expression tag
Ser 616	-	expression tag
His 617	-	expression tag
Pro 618	-	expression tag
Gln 619	-	expression tag
Phe 620	-	expression tag
Glu 621	-	expression tag
Lys 622	-	expression tag
Gly 623	-	expression tag
Ser 624	-	expression tag
Gly 625	-	expression tag
Gly 626	-	expression tag
Leu 627	-	expression tag
Asn 628	-	expression tag
Asp 629	-	expression tag
Ile 630	-	expression tag
Phe 631	-	expression tag
Glu 632	-	expression tag
Ala 633	-	expression tag
Gln 634	-	expression tag
Lys 635	-	expression tag
Ile 636	-	expression tag
Glu 637	-	expression tag
Trp 638	-	expression tag
His 639	-	expression tag
Glu 640	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	203063.8
Branched	Number of molecules	18
	Total formula weight	7639.2
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	212030.3

*Water molecules are not included.