8DMH

Lymphocytic choriomeningitis virus glycoprotein in complex with neutralizing antibody M28

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I (A, B, C)	Glycoprotein G1	polymer	225	25521.2	3	UniProt (P09991) Pfam (PF00798)	Lymphocytic choriomeningitis virus
2	B, F, J (E, G, I)	18.5C-M28 Fab Light Chain	polymer	214	23306.8	3		Homo sapiens (human)
3	C, G, K (F, H, J)	18.5C-M28 Fab Heavy Chain	polymer	220	23610.7	3		Homo sapiens (human)
4	D, H, L (a, b, c)	Glycoprotein G2,Cobalamin adenosyltransferase-like domain-containing protein trimerization tag	polymer	375	42166.8	3	UniProt (P09991) UniProt (Q9HIA7) Pfam (PF00798) Pfam (PF01923)	Lymphocytic choriomeningitis virus	GP2
5	M, S, Y (D, P, V)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
6	AA, BA, CA, DA, N... (X, Y, Z, d, K...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	15	In PDB GlyTouCan (G42666HT)
7	EA, FA, GA, HA, IA... (A, a, B, b, C...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A, B, C: 58 - 265 (UniProt: P09991)

PDB	External Database	Details
Met 41	-	initiating methionine
Lys 42	-	expression tag
Leu 43	-	expression tag
Cys 44	-	expression tag
Ile 45	-	expression tag
Leu 46	-	expression tag
Leu 47	-	expression tag
Ala 48	-	expression tag
Val 49	-	expression tag
Val 50	-	expression tag
Ala 51	-	expression tag
Phe 52	-	expression tag
Val 53	-	expression tag
Gly 54	-	expression tag
Leu 55	-	expression tag
Ser 56	-	expression tag
Leu 57	-	expression tag
Cys 207	Gly 207	engineered mutation
Leu 260	Phe 260	conflict
Arg 264	Leu 264	engineered mutation
Arg 265	Ala 265	engineered mutation

a, b, c: 266 - 430 (UniProt: P09991)

PDB	External Database	Details
Glu 313	Ala 313	variant
Pro 334	Glu 334	engineered mutation
Cys 366	Gly 366	engineered mutation
Ala 398	Ser 398	engineered mutation
Leu 431	-	linker
Glu 432	-	linker
Gly 433	-	linker
Gly 434	-	linker

a, b, c: 435 - 583 (UniProt: Q9HIA7)

PDB	External Database	Details
Val 522	Ile 110	conflict
Ile 571	Leu 159	conflict
Ala 584	-	expression tag
Leu 585	-	expression tag
Glu 586	-	expression tag
Val 587	-	expression tag
Asp 588	-	expression tag
Asp 589	-	expression tag
Asp 590	-	expression tag
Asp 591	-	expression tag
Lys 592	-	expression tag
Ala 593	-	expression tag
Gly 594	-	expression tag
Trp 595	-	expression tag
Ser 596	-	expression tag
His 597	-	expression tag
Pro 598	-	expression tag
Gln 599	-	expression tag
Phe 600	-	expression tag
Glu 601	-	expression tag
Lys 602	-	expression tag
Gly 603	-	expression tag
Gly 604	-	expression tag
Gly 605	-	expression tag
Ser 606	-	expression tag
Gly 607	-	expression tag
Gly 608	-	expression tag
Gly 609	-	expression tag
Ser 610	-	expression tag
Gly 611	-	expression tag
Gly 612	-	expression tag
Gly 613	-	expression tag
Ser 614	-	expression tag
Trp 615	-	expression tag
Ser 616	-	expression tag
His 617	-	expression tag
Pro 618	-	expression tag
Gln 619	-	expression tag
Phe 620	-	expression tag
Glu 621	-	expression tag
Lys 622	-	expression tag
Gly 623	-	expression tag
Ser 624	-	expression tag
Gly 625	-	expression tag
Gly 626	-	expression tag
Leu 627	-	expression tag
Asn 628	-	expression tag
Asp 629	-	expression tag
Ile 630	-	expression tag
Phe 631	-	expression tag
Glu 632	-	expression tag
Ala 633	-	expression tag
Gln 634	-	expression tag
Lys 635	-	expression tag
Ile 636	-	expression tag
Glu 637	-	expression tag
Trp 638	-	expression tag
His 639	-	expression tag
Glu 640	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	343816.5
Branched	Number of molecules	18
	Total formula weight	8125.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	353269.3

*Water molecules are not included.