8DK3

CryoEM structure of Pseudomonas aeruginosa PA14 JetC ATPase domain bound to DNA and cWHD domain of JetA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	JetC	polymer	1119	126683.2	2	UniProt (A0A8G4Z850) In PDB	Pseudomonas aeruginosa PA14
2	C	JetA	polymer	517	59233.5	1	UniProt (A0A0H2ZJP9) Pfam (PF11855) In PDB	Pseudomonas aeruginosa PA14
3	P	DNA (26-MER)	polymer	26	7864.1	1		synthetic construct
4	Q	DNA (26-MER)	polymer	26	8098.4	1		synthetic construct
5	A, B	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	2	Chemie (AGS)
6	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B: 2 - 1101 (UniProt: A0A8G4Z850)

PDB	External Database	Details
Met -17	-	initiating methionine
Lys -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Asn 0	-	expression tag
Ala 1	-	expression tag
Gln 1022	Glu 1022	conflict

C: -5 - 499 (UniProt: A0A0H2ZJP9)

PDB	External Database	Details
Met -17	-	initiating methionine
Lys -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Tyr -4	Trp 35	conflict
Phe -3	Lys 36	conflict
Gln -2	Val 37	conflict
Ser -1	Ala 38	conflict
Asn 0	Ala 39	conflict
Ala 1	Met 40	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	328562.3
Non-Polymers*	Number of molecules	4
	Total formula weight	1095.1
All*	Total formula weight	329657.4

*Water molecules are not included.