8DK2

CryoEM structure of Pseudomonas aeruginosa PA14 JetABC in an unclamped state trapped in ATP dependent dimeric form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	JetA	polymer	517	59233.5	2	UniProt (A0A0H2ZJP9) Pfam (PF11855) In PDB	Pseudomonas aeruginosa PA14
2	C, D	JetC	polymer	1119	126683.2	2	UniProt (A0A8G4Z850) In PDB	Pseudomonas aeruginosa PA14
3	F, G, H, I	JetB	polymer	249	27537.0	4	UniProt (A0A0H2ZL66) Pfam (PF13835) In PDB	Pseudomonas aeruginosa PA14
4	P	DNA (26-MER)	polymer	26	7864.1	1		synthetic construct
5	Q	DNA (26-MER)	polymer	26	8098.4	1		synthetic construct
6	C, D	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	2	Chemie (AGS)
7	C, D	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B: -5 - 499 (UniProt: A0A0H2ZJP9)

PDB	External Database	Details
Met -17	-	initiating methionine
Lys -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Tyr -4	Trp 35	conflict
Phe -3	Lys 36	conflict
Gln -2	Val 37	conflict
Ser -1	Ala 38	conflict
Asn 0	Ala 39	conflict
Ala 1	Met 40	conflict

C, D: 2 - 1101 (UniProt: A0A8G4Z850)

PDB	External Database	Details
Met -17	-	initiating methionine
Lys -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Asn 0	-	expression tag
Ala 1	-	expression tag
Gln 1022	Glu 1022	conflict

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	497944.0
Non-Polymers*	Number of molecules	4
	Total formula weight	1095.1
All*	Total formula weight	499039.1

*Water molecules are not included.