8DGT
Cryo-EM structure of a RAS/RAF complex (state 2)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Serine/threonine-protein kinase B-raf | polymer | 805 | 89402.8 | 1 | UniProt (P15056) Pfam (PF02196) Pfam (PF00130) Pfam (PF07714) In PDB | Homo sapiens (human) | Proto-oncogene B-Raf,p94,v-Raf murine sarcoma viral oncogene homolog B1 |
2 | B | Dual specificity mitogen-activated protein kinase kinase 1 | polymer | 414 | 45835.4 | 1 | UniProt (Q02750) Pfam (PF00069) In PDB | Homo sapiens (human) | MKK1,ERK activator kinase 1,MAPK/ERK kinase 1,MEK 1 |
3 | C, D | 14-3-3 protein zeta | polymer | 247 | 28108.5 | 2 | UniProt (V9P4T4) Pfam (PF00244) In PDB | Spodoptera exigua (beet armyworm) | |
4 | F | GTPase KRas isoform X2 | polymer | 191 | 21868.6 | 1 | UniProt (A0A6P5IP77) Pfam (PF00071) UniProt (by SIFTS) (P01116) In PDB | Homo sapiens (human) | |
5 | A, B | PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER | non-polymer | 523.2 | 2 | Chemie (AGS) | |||
6 | A | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
7 | B, F | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
8 | B | 5-[(2-fluoro-4-iodophenyl)amino]-N-(2-hydroxyethoxy)imidazo[1,5-a]pyridine-6-carboxamide | non-polymer | 456.2 | 1 | Chemie (LCJ) | |||
9 | F | PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | non-polymer | 522.2 | 1 | Chemie (GNP) |
Sequence modifications
A: 1 - 766 (UniProt: P15056)
B: 1 - 392 (UniProt: Q02750)
F: 1 - 169 (UniProt: A0A6P5IP77)
PDB | External Database | Details |
---|---|---|
Met -26 | - | expression tag |
Ser -25 | - | expression tag |
Tyr -24 | - | expression tag |
Tyr -23 | - | expression tag |
His -22 | - | expression tag |
His -21 | - | expression tag |
His -20 | - | expression tag |
His -19 | - | expression tag |
His -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
Asp -14 | - | expression tag |
Ile -13 | - | expression tag |
Pro -12 | - | expression tag |
Thr -11 | - | expression tag |
Thr -10 | - | expression tag |
Glu -9 | - | expression tag |
Asn -8 | - | expression tag |
Leu -7 | - | expression tag |
Tyr -6 | - | expression tag |
Phe -5 | - | expression tag |
Gln -4 | - | expression tag |
Gly -3 | - | expression tag |
Ala -2 | - | expression tag |
Met -1 | - | expression tag |
Asp 0 | - | expression tag |
Gly 767 | - | expression tag |
Thr 768 | - | expression tag |
Ser 769 | - | expression tag |
Ala 770 | - | expression tag |
Trp 771 | - | expression tag |
Ser 772 | - | expression tag |
His 773 | - | expression tag |
Pro 774 | - | expression tag |
Gln 775 | - | expression tag |
Phe 776 | - | expression tag |
Glu 777 | - | expression tag |
Lys 778 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met -21 | - | expression tag |
Gly -20 | - | expression tag |
Ser -19 | - | expression tag |
Ser -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
Ser -11 | - | expression tag |
Ala -10 | - | expression tag |
Val -9 | - | expression tag |
Asp -8 | - | expression tag |
Glu -7 | - | expression tag |
Asn -6 | - | expression tag |
Leu -5 | - | expression tag |
Tyr -4 | - | expression tag |
Phe -3 | - | expression tag |
Gln -2 | - | expression tag |
Gly -1 | - | expression tag |
Gly 0 | - | expression tag |
Ala 218 | Ser 218 | engineered mutation |
Ala 222 | Ser 222 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -21 | - | initiating methionine |
His -20 | - | expression tag |
His -19 | - | expression tag |
His -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
Gly -14 | - | expression tag |
Ser -13 | - | expression tag |
Leu -12 | - | expression tag |
Val -11 | - | expression tag |
Pro -10 | - | expression tag |
Arg -9 | - | expression tag |
Ser -8 | - | expression tag |
Glu -7 | - | expression tag |
Asn -6 | - | expression tag |
Leu -5 | - | expression tag |
Tyr -4 | - | expression tag |
Phe -3 | - | expression tag |
Gln -2 | - | expression tag |
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 213323.9 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 2204.3 | |
All* | Total formula weight | 215528.2 |