8DGT

Cryo-EM structure of a RAS/RAF complex (state 2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Serine/threonine-protein kinase B-raf	polymer	805	89402.8	1	UniProt (P15056) Pfam (PF02196) Pfam (PF00130) Pfam (PF07714)	Homo sapiens (human)	Proto-oncogene B-Raf,p94,v-Raf murine sarcoma viral oncogene homolog B1
2	B (B)	Dual specificity mitogen-activated protein kinase kinase 1	polymer	414	45835.4	1	UniProt (Q02750) Pfam (PF00069)	Homo sapiens (human)	MKK1,ERK activator kinase 1,MAPK/ERK kinase 1,MEK 1
3	C, D (C, D)	14-3-3 protein zeta	polymer	247	28108.5	2	UniProt (V9P4T4) Pfam (PF00244)	Spodoptera exigua (beet armyworm)
4	E (F)	GTPase KRas isoform X2	polymer	191	21868.6	1	UniProt (A0A6P5IP77) Pfam (PF00071) UniProt (by SIFTS) (P01116)	Homo sapiens (human)
5	F, J (A, B)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	2	Chemie (AGS)
6	G, H (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	I, L (B, F)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
8	K (B)	5-[(2-fluoro-4-iodophenyl)amino]-N-(2-hydroxyethoxy)imidazo[1,5-a]pyridine-6-carboxamide	non-polymer		456.2	1	Chemie (LCJ)
9	M (F)	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	1	Chemie (GNP)

Sequence modifications

A: 1 - 766 (UniProt: P15056)

PDB	External Database	Details
Met -26	-	expression tag
Ser -25	-	expression tag
Tyr -24	-	expression tag
Tyr -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Asp -14	-	expression tag
Ile -13	-	expression tag
Pro -12	-	expression tag
Thr -11	-	expression tag
Thr -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Asp 0	-	expression tag
Gly 767	-	expression tag
Thr 768	-	expression tag
Ser 769	-	expression tag
Ala 770	-	expression tag
Trp 771	-	expression tag
Ser 772	-	expression tag
His 773	-	expression tag
Pro 774	-	expression tag
Gln 775	-	expression tag
Phe 776	-	expression tag
Glu 777	-	expression tag
Lys 778	-	expression tag

B: 1 - 392 (UniProt: Q02750)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ala -10	-	expression tag
Val -9	-	expression tag
Asp -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag
Ala 218	Ser 218	engineered mutation
Ala 222	Ser 222	engineered mutation

F: 1 - 169 (UniProt: A0A6P5IP77)

PDB	External Database	Details
Met -21	-	initiating methionine
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Gly -14	-	expression tag
Ser -13	-	expression tag
Leu -12	-	expression tag
Val -11	-	expression tag
Pro -10	-	expression tag
Arg -9	-	expression tag
Ser -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	213323.9
Non-Polymers*	Number of molecules	8
	Total formula weight	2204.3
All*	Total formula weight	215528.2

*Water molecules are not included.