8DF9

Structure of M. kandleri topoisomerase V in complex with DNA. 38 base pair asymmetric DNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Topoisomerase V	polymer	854	97110.4	2	UniProt (Q977W1) Pfam (PF13412) Pfam (PF21872) Pfam (PF21616) Pfam (PF14520) Pfam (PF22409)	Methanopyrus kandleri	Winged helix-turn-helix transcriptional regulator
2	C (S)	DNA (33-MER)	polymer	33	10203.6	1		synthetic construct
3	D (T)	DNA (33-MER)	polymer	33	10096.5	1		synthetic construct
4	E (U)	DNA (5'-D(P*GP*CP*CP*TP*GP*CP*AP*CP*GP*AP*AP*GP*TP*AP*AP*GP*C)-3')	polymer	17	5221.4	1		synthetic construct
5	F, H (V, X)	DNA (5'-D(P*TP*GP*CP*TP*TP*AP*CP*TP*TP*CP*GP*TP*GP*CP*AP*GP*GP*CP*CP*A)-3')	polymer	20	6116.0	2		synthetic construct
6	G (W)	DNA (5'-D(P*GP*CP*CP*TP*GP*CP*AP*CP*GP*AP*AP*GP*TP*AP*AP*GP*CP*A)-3')	polymer	18	5534.6	1		synthetic construct
7	I, J, K, L, M... (A, B)	MAGNESIUM ION	non-polymer		24.3	12	Chemie (MG)
8	U, V (S, T)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
9	W, X (A, B)	water	water		18.0	30	Chemie (HOH)

Sequence modifications

A, B: 1 - 854 (UniProt: Q977W1)

PDB	External Database	Details
Ala 809	Lys 809	engineered mutation
Ala 820	Lys 820	engineered mutation
Ala 831	Lys 831	engineered mutation
Ala 835	Lys 835	engineered mutation
Ala 846	Lys 846	engineered mutation
Ala 851	Lys 851	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	237508.9
Non-Polymers*	Number of molecules	14
	Total formula weight	369.9
All*	Total formula weight	237878.7

*Water molecules are not included.