8DD7

The Cryo-EM structure of Drosophila Cryptochrome in complex with Timeless

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Methylated-DNA--protein-cysteine methyltransferase,Cryptochrome-1 fusion	polymer	746	83838.6	1	UniProt (E5BBQ0) UniProt (O77059) Pfam (PF00875) Pfam (PF03441)	Homo sapiens (human)
2	B (B)	Protein timeless,Methylated-DNA--protein-cysteine methyltransferase fusion	polymer	1618	179996.1	1	UniProt (P49021) UniProt (E5BBQ0) Pfam (PF04821) Pfam (PF05029)	Drosophila melanogaster (fruit fly)	6-O-methylguanine-DNA methyltransferase,O-6-methylguanine-DNA-alkyltransferase
3	C (A)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	1	Chemie (FAD)

Sequence modifications

A: -188 - -8 (UniProt: E5BBQ0)

PDB	External Database	Details
Met -225	-	initiating methionine
Glu -224	-	expression tag
Gln -223	-	expression tag
Lys -222	-	expression tag
Leu -221	-	expression tag
Ile -220	-	expression tag
Ser -219	-	expression tag
Glu -218	-	expression tag
Glu -217	-	expression tag
Asp -216	-	expression tag
Leu -215	-	expression tag
Gly -214	-	expression tag
Gly -213	-	expression tag
Gly -212	-	expression tag
Glu -211	-	expression tag
Gln -210	-	expression tag
Lys -209	-	expression tag
Leu -208	-	expression tag
Ile -207	-	expression tag
Ser -206	-	expression tag
Glu -205	-	expression tag
Glu -204	-	expression tag
Asp -203	-	expression tag
Leu -202	-	expression tag
Gly -201	-	expression tag
Gly -200	-	expression tag
Gly -199	-	expression tag
Glu -198	-	expression tag
Gln -197	-	expression tag
Lys -196	-	expression tag
Leu -195	-	expression tag
Ile -194	-	expression tag
Ser -193	-	expression tag
Glu -192	-	expression tag
Glu -191	-	expression tag
Asp -190	-	expression tag
Leu -189	-	expression tag
Gly -187	Pro 2	conflict
Met -185	Ser 4	conflict
Arg -156	Glu 33	conflict
Ile -126	Met 63	conflict
Glu -72	Tyr 117	conflict
Val -65	Ala 124	conflict
Asn -55	Lys 134	conflict
Asp -51	Ser 138	conflict
Ser -33	Leu 156	conflict
Pro -29	Gly 160	conflict
Leu -27	Glu 162	conflict
Pro -7	-	linker
Gly -6	-	linker
Leu -5	-	linker
Gly -4	-	linker
Gly -3	-	linker
Gly -2	-	linker
Ser -1	-	linker
Gly 0	-	linker

A: 1 - 520 (UniProt: O77059)
B: 1 - 1398 (UniProt: P49021)

PDB	External Database	Details
Leu 1399	-	linker
Glu 1400	-	linker
Gly 1401	-	linker
Gly 1402	-	linker
Ser 1403	-	linker
Gly 1404	-	linker
Met 1405	-	linker

B: 1406 - 1582 (UniProt: E5BBQ0)

PDB	External Database	Details
Arg 1434	Glu 33	conflict
Pro 1583	-	expression tag
Gly 1584	-	expression tag
Leu 1585	-	expression tag
Gly 1586	-	expression tag
Gly 1587	-	expression tag
Gly 1588	-	expression tag
Gly 1589	-	expression tag
Tyr 1590	-	expression tag
Pro 1591	-	expression tag
Tyr 1592	-	expression tag
Asp 1593	-	expression tag
Val 1594	-	expression tag
Pro 1595	-	expression tag
Asp 1596	-	expression tag
Tyr 1597	-	expression tag
Ala 1598	-	expression tag
Arg 1599	-	expression tag
Thr 1600	-	expression tag
Gly 1601	-	expression tag
Gly 1602	-	expression tag
Gly 1603	-	expression tag
Ser 1604	-	expression tag
Gly 1605	-	expression tag
Ser 1606	-	expression tag
Arg 1607	-	expression tag
Leu 1608	-	expression tag
Glu 1609	-	expression tag
Glu 1610	-	expression tag
Glu 1611	-	expression tag
Leu 1612	-	expression tag
Arg 1613	-	expression tag
Arg 1614	-	expression tag
Arg 1615	-	expression tag
Leu 1616	-	expression tag
Thr 1617	-	expression tag
Glu 1618	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	263834.7
Non-Polymers*	Number of molecules	1
	Total formula weight	785.5
All*	Total formula weight	264620.3

*Water molecules are not included.