8DCD

RNA ligase RtcB from Pyrococcus horikoshii in complex with Zn2+ and GTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	tRNA-splicing ligase RtcB	polymer	501	55753.8	2	UniProt (O59245) Pfam (PF01139)	Pyrococcus horikoshii OT3	3'-phosphate/5'-hydroxy nucleic acid ligase,tRNA ligase RtcB
2	C, D (C, D)	beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900003)
3	E, U (A, B)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
4	F, V (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	AA, BA, G, H, I... (B, A)	SULFATE ION	non-polymer		96.1	16	Chemie (SO4)
6	CA, DA, Q, R (B, A)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
7	S, T (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
8	EA, FA (A, B)	water	water		18.0	160	Chemie (HOH)

Sequence modifications

A, B: 1 - 96 (UniProt: O59245)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

A, B: 97 - 481 (UniProt: O59245)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	111507.6
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	26
	Total formula weight	3100.1
All*	Total formula weight	115292.3

*Water molecules are not included.