8DCB

RNA ligase RtcB from Pyrococcus horikoshii in complex with Ni2+ and GTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	tRNA-splicing ligase RtcB	polymer	501	55753.8	2	UniProt (O59245) Pfam (PF01139) In PDB	Pyrococcus horikoshii OT3	3'-phosphate/5'-hydroxy nucleic acid ligase,tRNA ligase RtcB
2	C, D	beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900003)
3	A, B	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
4	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	A, B	NICKEL (II) ION	non-polymer		58.7	5	Chemie (NI)
6	A, B	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
7	A, B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
8		water	water		18.0	17	Chemie (HOH)

Sequence modifications

A, B: 1 - 96 (UniProt: O59245)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

A, B: 97 - 481 (UniProt: O59245)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	111507.6
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	20
	Total formula weight	2463.5
All*	Total formula weight	114655.6

*Water molecules are not included.