8DAW

Saccharomyces cerevisiae Ufd1/Npl4/Cdc48 complex bound to three ubiquitin moieties and one unfolded ubiquitin in presence of SUMO-ubiquitin(K48polyUb)-mEOS and ATP, state 2 (uD)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Cell division control protein 48	polymer	838	92389.2	6	UniProt (P25694) Pfam (PF02359) Pfam (PF02933) Pfam (PF00004) Pfam (PF17862) In PDB	Saccharomyces cerevisiae (baker's yeast)	Cell division cycle protein 48,Transitional endoplasmic reticulum ATPase homolog
2	G	Nuclear protein localization protein 4	polymer	583	66144.3	1	UniProt (P33755) Pfam (PF05020) Pfam (PF05021) In PDB	Saccharomyces cerevisiae (baker's yeast)	HMG-CoA reductase degradation protein 4
3	H	Ubiquitin fusion degradation protein 1	polymer	363	40001.4	1	UniProt (P53044) Pfam (PF03152) In PDB	Saccharomyces cerevisiae (baker's yeast)	UB fusion protein 1,Polymerase-interacting protein 3
4	I, J, K, L	Ubiquitin	polymer	76	8568.8	4	UniProt (P0CG63) Pfam (PF00240) In PDB	Saccharomyces cerevisiae (baker's yeast)
5	A, B, C, D, F	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	5	Chemie (ATP)
6	A, B, C, D, E...	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	7	Chemie (ADP)
7	G	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, B, C, D, E, F: 1 - 835 (UniProt: P25694)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

G: 1 - 580 (UniProt: P33755)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

H: 1 - 361 (UniProt: P53044)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	694756.0
Non-Polymers*	Number of molecules	14
	Total formula weight	5657.1
All*	Total formula weight	700413.2

*Water molecules are not included.