8DA9

Coevolved affibody-Z domain pair LL2.c3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Immunoglobulin G-binding protein A	polymer	67	7815.6	2	UniProt (P38507) Pfam (PF02216) UniProt (by SIFTS) (P02976) In PDB	Staphylococcus aureus	IgG-binding protein A,Staphylococcal protein A,SpA
2	B	Affibody LL2.FIIV	polymer	67	7643.6	1		synthetic construct
3	D	Affibody LL2.FIIV	polymer	67	7670.6	1		synthetic construct
4	A, C	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	A, C, D	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6		water	water		18.0	134	Chemie (HOH)

Sequence modifications

A, C: 2 - 58 (UniProt: P38507)

PDB	External Database	Details
Ala 0	-	expression tag
Val 1	-	expression tag
Leu 9	Gln 220	engineered mutation
Val 13	Phe 224	engineered mutation
Ala 29	Gly 240	engineered mutation
Phe 31	Ile 242	engineered mutation
Leu 59	-	expression tag
Glu 60	-	expression tag
His 61	-	expression tag
His 62	-	expression tag
His 63	-	expression tag
His 64	-	expression tag
His 65	-	expression tag
His 66	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	30945.5
Non-Polymers*	Number of molecules	5
	Total formula weight	472.4
All*	Total formula weight	31417.9

*Water molecules are not included.