8D9L

CryoEM structure of human METTL1-WDR4 in complex with Lys-tRNA and SAM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4	polymer	405	45123.0	1	UniProt (P57081) Pfam (PF00400) In PDB	Homo sapiens (human)	Protein Wuho homolog,hWH,WD repeat-containing protein 4
2	A	tRNA (guanine-N(7)-)-methyltransferase	polymer	276	31472.0	1	UniProt (Q9UBP6) Pfam (PF02390) In PDB	Homo sapiens (human)	Methyltransferase-like protein 1,mRNA (guanine-N(7)-)-methyltransferase,miRNA (guanine-N(7)-)-methyltransferase,tRNA (guanine(46)-N(7))-methyltransferase,tRNA(m7G46)-methyltransferase
3	C	Lys-tRNA	polymer	73	23507.9	1	GenBank (1781322811)	Homo sapiens
4	A	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)

Sequence modifications

B: 1 - 389 (UniProt: P57081)

PDB	External Database	Details
Met -15	-	initiating methionine
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

A: 1 - 276 (UniProt: Q9UBP6)

PDB	External Database	Details
Ala 163	Asp 163	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	100102.9
Non-Polymers*	Number of molecules	1
	Total formula weight	398.4
All*	Total formula weight	100501.4

*Water molecules are not included.