8D9L

CryoEM structure of human METTL1-WDR4 in complex with Lys-tRNA and SAM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4	polymer	405	45123.0	1	UniProt (P57081) Pfam (PF00400)	Homo sapiens (human)	Protein Wuho homolog,hWH,WD repeat-containing protein 4
2	B (A)	tRNA (guanine-N(7)-)-methyltransferase	polymer	276	31472.0	1	UniProt (Q9UBP6) Pfam (PF02390)	Homo sapiens (human)	Methyltransferase-like protein 1,mRNA (guanine-N(7)-)-methyltransferase,miRNA (guanine-N(7)-)-methyltransferase,tRNA (guanine(46)-N(7))-methyltransferase,tRNA(m7G46)-methyltransferase
3	C (C)	Lys-tRNA	polymer	73	23507.9	1	GenBank (1781322811)	Homo sapiens
4	D (A)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM) PubChem (5136) PubChem (34755) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (9865603) PubChem (145712080) PubChem (12312798) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094)

Sequence modifications

B: 1 - 389 (UniProt: P57081)

PDB	External Database	Details
Met -15	-	initiating methionine
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

A: 1 - 276 (UniProt: Q9UBP6)

PDB	External Database	Details
Ala 163	Asp 163	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	100102.9
Non-Polymers*	Number of molecules	1
	Total formula weight	398.4
All*	Total formula weight	100501.4

*Water molecules are not included.