8D4F
beta-Arf1 mediated dimeric assembly of AP-1, Arf1, Nef complex within lattice on MHC-I lipopeptide incorporated wide(r) membrane tubes
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, K (B, A) | AP-1 complex subunit beta-1 | polymer | 949 | 104736.5 | 2 | UniProt (Q10567) Pfam (PF01602) Pfam (PF02883) Pfam (PF09066) | Homo sapiens (human) | Adaptor protein complex AP-1 subunit beta-1,Adaptor-related protein complex 1 subunit beta-1,Beta-1-adaptin,Beta-adaptin 1,Clathrin assembly protein complex 1 beta large chain,Golgi adaptor HA1/AP1 adaptin beta subunit |
| 2 | B, D, J, L, N... (C, H, Z, D, F...) | ADP-ribosylation factor 1 | polymer | 180 | 20590.5 | 6 | UniProt (P84077) Pfam (PF00025) | Homo sapiens (human) | |
| 3 | C, M (G, E) | AP-1 complex subunit gamma-1 | polymer | 601 | 68194.1 | 2 | UniProt (P22892) Pfam (PF01602) | Mus musculus (house mouse) | Adaptor protein complex AP-1 subunit gamma-1,Adaptor-related protein complex 1 subunit gamma-1,Clathrin assembly protein complex 1 gamma-1 large chain,Gamma-adaptin,Gamma1-adaptin,Golgi adaptor HA1/AP1 adaptin subunit gamma-1 |
| 4 | E, G, O, Q (L, N, I, K) | Protein Nef | polymer | 213 | 24285.2 | 4 | UniProt (Q90VU7) | Human immunodeficiency virus 1 | 3'ORF,Negative factor,F-protein |
| 5 | F, P (M, J) | AP-1 complex subunit mu-1 | polymer | 423 | 48606.7 | 2 | UniProt (P35585) Pfam (PF01217) Pfam (PF00928) | Mus musculus (house mouse) | AP-mu chain family member mu1A,Adaptor protein complex AP-1 subunit mu-1,Adaptor-related protein complex 1 subunit mu-1,Clathrin assembly protein complex 1 mu-1 medium chain 1,Clathrin coat assembly protein AP47,Clathrin coat-associated protein AP47,Golgi adaptor HA1/AP1 adaptin mu-1 subunit,Mu-adaptin 1,Mu1A-adaptin |
| 6 | H, R (S, O) | AP-1 complex subunit sigma-3 | polymer | 154 | 18305.3 | 2 | UniProt (Q96PC3) Pfam (PF01217) | Homo sapiens (human) | Adaptor protein complex AP-1 subunit sigma-1C,Adaptor-related protein complex 1 subunit sigma-1C,Clathrin assembly protein complex 1 sigma-1C small chain,Golgi adaptor HA1/AP1 adaptin sigma-1C subunit,Sigma 1C subunit of AP-1 clathrin,Sigma-adaptin 1C,Sigma1C-adaptin |
| 7 | I, S (Y, P) | HLA class I histocompatibility antigen, A alpha chain | polymer | 39 | 4139.4 | 2 | UniProt (P04439) Pfam (PF06623) | Homo sapiens (human) | Human leukocyte antigen A,HLA-A |
| 8 | AA, CA, EA, U, W... (D, F, Q, C, H...) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 6 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 9 | BA, DA, FA, V, X... (D, F, Q, C, H...) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) PubChem (888) |
Sequence modifications
B, A: 1 - 949 (UniProt: Q10567)
G, E: 1 - 595 (UniProt: P22892)
L, N, I, K: 1 - 206 (UniProt: Q90VU7)
Y, P: 334 - 365 (UniProt: P04439)
| PDB | External Database | Details |
|---|---|---|
| Arg 359 | Lys 359 | engineered mutation |
| Lys 476 | Glu 476 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Glu 596 | - | expression tag |
| Asn 597 | - | expression tag |
| Leu 598 | - | expression tag |
| Tyr 599 | - | expression tag |
| Phe 600 | - | expression tag |
| Gln 601 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 207 | - | expression tag |
| His 208 | - | expression tag |
| His 209 | - | expression tag |
| His 210 | - | expression tag |
| His 211 | - | expression tag |
| His 212 | - | expression tag |
| His 213 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Cys 333 | - | expression tag |
| Ser 345 | Thr 345 | engineered mutation |
| Gly 349 | Ser 349 | engineered mutation |
| Ser 355 | Gly 355 | engineered mutation |
| Ala 363 | Cys 363 | engineered mutation |
| His 366 | - | expression tag |
| His 367 | - | expression tag |
| His 368 | - | expression tag |
| His 369 | - | expression tag |
| His 370 | - | expression tag |
| His 371 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 20 |
| Total formula weight | 708648.2 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 3284.9 | |
| All* | Total formula weight | 711933.1 |
