8D4C

beta-Arf1 mediated dimeric assembly of AP-1, Arf1, Nef complex within lattice on MHC-I lipopeptide incorporated narrow membrane tubes

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, J, K (C, H, D, F)	ADP-ribosylation factor 1	polymer	180	20590.5	4	UniProt (P84077) Pfam (PF00025)	Homo sapiens (human)
2	C, G, L, P (N, L, K, I)	Protein Nef	polymer	212	24154.0	4	UniProt (Q90VU7) Pfam (PF00469)	Human immunodeficiency virus 1	3'ORF,Negative factor,F-protein
3	D, M (Y, P)	HLA class I histocompatibility antigen, A alpha chain	polymer	39	4139.4	2	UniProt (P04439) Pfam (PF06623)	Homo sapiens (human)	Human leukocyte antigen A,HLA-A
4	E, N (B, A)	AP-1 complex subunit beta-1	polymer	948	104605.3	2	UniProt (Q10567) Pfam (PF01602) Pfam (PF02883) Pfam (PF09066)	Homo sapiens (human)	Adaptor protein complex AP-1 subunit beta-1,Adaptor-related protein complex 1 subunit beta-1,Beta-1-adaptin,Beta-adaptin 1,Clathrin assembly protein complex 1 beta large chain,Golgi adaptor HA1/AP1 adaptin beta subunit
5	F, O (G, E)	AP-1 complex subunit gamma-1	polymer	600	68062.9	2	UniProt (P22892) Pfam (PF01602)	Mus musculus (house mouse)	Adaptor protein complex AP-1 subunit gamma-1,Adaptor-related protein complex 1 subunit gamma-1,Clathrin assembly protein complex 1 gamma-1 large chain,Gamma-adaptin,Gamma1-adaptin,Golgi adaptor HA1/AP1 adaptin subunit gamma-1
6	H, Q (M, J)	AP-1 complex subunit mu-1	polymer	423	48606.7	2	UniProt (P35585) Pfam (PF01217) Pfam (PF00928)	Mus musculus (house mouse)	AP-mu chain family member mu1A,Adaptor protein complex AP-1 subunit mu-1,Adaptor-related protein complex 1 subunit mu-1,Clathrin assembly protein complex 1 mu-1 medium chain 1,Clathrin coat assembly protein AP47,Clathrin coat-associated protein AP47,Golgi adaptor HA1/AP1 adaptin mu-1 subunit,Mu-adaptin 1,Mu1A-adaptin
7	I, R (S, O)	AP-1 complex subunit sigma-3	polymer	154	18305.3	2	UniProt (Q96PC3) Pfam (PF01217)	Homo sapiens (human)	Adaptor protein complex AP-1 subunit sigma-1C,Adaptor-related protein complex 1 subunit sigma-1C,Clathrin assembly protein complex 1 sigma-1C small chain,Golgi adaptor HA1/AP1 adaptin sigma-1C subunit,Sigma 1C subunit of AP-1 clathrin,Sigma-adaptin 1C,Sigma1C-adaptin
8	S, U, W, Y (C, H, D, F)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	4	Chemie (GTP)
9	T, V, X, Z (C, H, D, F)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)

Sequence modifications

N, L, K, I: 2 - 206 (UniProt: Q90VU7)

PDB	External Database	Details
Gly 207	-	expression tag
His 208	-	expression tag
His 209	-	expression tag
His 210	-	expression tag
His 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag

Y, P: 334 - 365 (UniProt: P04439)

PDB	External Database	Details
Cys 333	-	expression tag
Ser 345	Thr 345	engineered mutation
Gly 349	Ser 349	engineered mutation
Ser 355	Gly 355	engineered mutation
Ala 363	Cys 363	engineered mutation
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag

B, A: 2 - 949 (UniProt: Q10567)

PDB	External Database	Details
Arg 359	Lys 359	engineered mutation
Lys 476	Glu 476	engineered mutation

G, E: 2 - 595 (UniProt: P22892)

PDB	External Database	Details
Glu 596	-	expression tag
Asn 597	-	expression tag
Leu 598	-	expression tag
Tyr 599	-	expression tag
Phe 600	-	expression tag
Gln 601	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	666417.6
Non-Polymers*	Number of molecules	8
	Total formula weight	2189.9
All*	Total formula weight	668607.5

*Water molecules are not included.