8D3Q

Type I-C Cas4-Cas1-Cas2 complex bound to a PAM/NoPAM prespacer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	CRISPR-associated endonuclease Cas1	polymer	343	39393.3	4	UniProt (Q9KFX9) Pfam (PF01867)	Alkalihalobacillus halodurans C-125
2	E, F (E, F)	CRISPR-associated endonuclease Cas2	polymer	98	11038.7	2	UniProt (Q9KFX8) Pfam (PF09827)	Alkalihalobacillus halodurans C-125
3	G (G)	PAM/NoPAM strand 2	polymer	28	8575.5	1		synthetic construct
4	H (H)	PAM/NoPAM strand 1	polymer	32	9840.4	1		synthetic construct
5	I, J (I, J)	CRISPR-associated exonuclease Cas4	polymer	218	25403.4	2	UniProt (A0A4Y7WTW2)	Alkalihalobacillus halodurans C-125
6	K, M (I, J)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
7	L (I)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)

Sequence modifications

E, F: 1 - 96 (UniProt: Q9KFX8)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

I, J: 3 - 219 (UniProt: A0A4Y7WTW2)

PDB	External Database	Details
Ala 2	-	expression tag
Met 11	Leu 11	conflict
Ser 101	Cys 101	conflict

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	248873.3
Non-Polymers*	Number of molecules	3
	Total formula weight	758.2
All*	Total formula weight	249631.6

*Water molecules are not included.