8D3Q
Type I-C Cas4-Cas1-Cas2 complex bound to a PAM/NoPAM prespacer
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | CRISPR-associated endonuclease Cas1 | polymer | 343 | 39393.3 | 4 | UniProt (Q9KFX9) Pfam (PF01867) In PDB | Alkalihalobacillus halodurans C-125 | |
2 | E, F | CRISPR-associated endonuclease Cas2 | polymer | 98 | 11038.7 | 2 | UniProt (Q9KFX8) Pfam (PF09827) In PDB | Alkalihalobacillus halodurans C-125 | |
3 | G | PAM/NoPAM strand 2 | polymer | 28 | 8575.5 | 1 | synthetic construct | ||
4 | H | PAM/NoPAM strand 1 | polymer | 32 | 9840.4 | 1 | synthetic construct | ||
5 | I, J | CRISPR-associated exonuclease Cas4 | polymer | 218 | 25403.4 | 2 | UniProt (A0A4Y7WTW2) Pfam (PF01930) In PDB | Alkalihalobacillus halodurans C-125 | |
6 | I, J | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
7 | I | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) |
Sequence modifications
E, F: 1 - 96 (UniProt: Q9KFX8)
I, J: 3 - 219 (UniProt: A0A4Y7WTW2)
PDB | External Database | Details |
---|---|---|
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ala 2 | - | expression tag |
Met 11 | Leu 11 | conflict |
Ser 101 | Cys 101 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 10 |
Total formula weight | 248873.3 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 758.2 | |
All* | Total formula weight | 249631.6 |