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8D3L

Type I-C Cas4-Cas1-Cas2 complex bound to a PAM/PAM prespacer

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A, B, C, D
(A, B, C, D)
CRISPR-associated endonuclease Cas1polymer34339393.34UniProt (Q9KFX9)
Pfam (PF01867)
Alkalihalobacillus halodurans C-125
2E, F
(E, F)
CRISPR-associated endonuclease Cas2polymer9811038.72UniProt (Q9KFX8)
Pfam (PF09827)
Alkalihalobacillus halodurans C-125
3G
(G)
PAM/PAM strand 2polymer3310139.51synthetic construct
4H
(H)
PAM/PAM strand 1polymer329840.41synthetic construct
5I, J
(I, J)
CRISPR-associated exonuclease Cas4polymer21825403.42UniProt (A0A4Y7WTW2)Alkalihalobacillus halodurans C-125
6K, M
(I, J)
IRON/SULFUR CLUSTERnon-polymer351.62Chemie (SF4)
7L, N
(I, J)
MANGANESE (II) IONnon-polymer54.92Chemie (MN)
8O, P, Q
(G, I, J)
waterwater18.04Chemie (HOH)
Sequence modifications
E, F: 1 - 96 (UniProt: Q9KFX8)
PDBExternal DatabaseDetails
Gly -1-expression tag
Ser 0-expression tag
I, J: 3 - 219 (UniProt: A0A4Y7WTW2)
PDBExternal DatabaseDetails
Ala 2-expression tag
Met 11Leu 11conflict
Ser 101Cys 101conflict
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains10
Total formula weight250437.3
Non-Polymers*Number of molecules4
Total formula weight813.2
All*Total formula weight251250.5
*Water molecules are not included.

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PDB entries from 2025-07-16

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