8D34

Crystal Structure of SARS CoV-2 NSP15 Endroribonuclease H250A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Uridylate-specific endoribonuclease nsp15	polymer	373	41821.4	2	UniProt (P0DTD1) Pfam (PF19219) Pfam (PF19216) Pfam (PF19215)	Severe acute respiratory syndrome coronavirus 2	NendoU,Non-structural protein 15,nsp15

Sequence modifications

A, B: 2 - 347 (UniProt: P0DTD1)

PDB	External Database	Details
Met -25	-	initiating methionine
Gly -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Val -12	-	expression tag
Asp -11	-	expression tag
Leu -10	-	expression tag
Gly -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Asn 0	-	expression tag
Met 1	-	expression tag
Ala 250	His 6701	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	83642.8
All*	Total formula weight	83642.8

*Water molecules are not included.