8D33

Human mitochondrial DNA polymerase gamma ternary complex with GC basepair

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase subunit gamma-1	polymer	1245	140559.5	1	UniProt (P54098) Pfam (PF18136) In PDB	Homo sapiens (human)	Mitochondrial DNA polymerase catalytic subunit,PolG-alpha
2	B, C	DNA polymerase subunit gamma-2, mitochondrial	polymer	491	55819.9	2	UniProt (Q9UHN1) Pfam (PF03129) In PDB	Homo sapiens (human)	DNA polymerase gamma accessory 55 kDa subunit,p55,Mitochondrial DNA polymerase accessory subunit,MtPolB,PolG-beta
3	P	DNA (5'-D(P*AP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*TP*AP*C)-3')	polymer	24	7357.8	1		synthetic construct
4	T	DNA (25-MER)	polymer	28	8644.5	1		synthetic construct
5	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
6	A	2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DCP)

Sequence modifications

A: 1 - 1239 (UniProt: P54098)

PDB	External Database	Details
His 1240	-	expression tag
His 1241	-	expression tag
His 1242	-	expression tag
His 1243	-	expression tag
His 1244	-	expression tag
His 1245	-	expression tag

B, C: 1 - 485 (UniProt: Q9UHN1)

PDB	External Database	Details
His 486	-	expression tag
His 487	-	expression tag
His 488	-	expression tag
His 489	-	expression tag
His 490	-	expression tag
His 491	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	268201.6
Non-Polymers*	Number of molecules	2
	Total formula weight	507.2
All*	Total formula weight	268708.8

*Water molecules are not included.