8D2K
Structure of Acidothermus cellulolyticus Cas9 ternary complex (Cleavage Intermediate 2)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease, Csn1 family | polymer | 1138 | 127498.1 | 1 | UniProt (A0LWB3) Pfam (PF18470) Pfam (PF01844) Pfam (PF18541) Pfam (PF17893) Pfam (PF17894) Pfam (PF18525) | Acidothermus cellulolyticus 11B | |
| 2 | B (B) | Single Guide RNA (102-MER) | polymer | 106 | 34390.3 | 1 | Acidothermus cellulolyticus | ||
| 3 | C (X) | DNA target strand (5'-D(*AP*GP*CP*TP*TP*GP*GP*TP*GP*TP*AP*TP*A)-3') | polymer | 13 | 4021.6 | 1 | Acidothermus cellulolyticus 11B | ||
| 4 | D (T) | DNA target strand (5'-D(P*CP*CP*AP*GP*GP*AP*TP*CP*TP*TP*GP*CP*CP*AP*TP*CP*CP*TP*AP*CP*CP*TP*CP*T)-3') | polymer | 24 | 7216.7 | 1 | Acidothermus cellulolyticus 11B | ||
| 5 | E (D) | DNA non-target strand (5'-D(P*TP*AP*TP*AP*CP*AP*CP*CP*AP*AP*GP*CP*T)-3') | polymer | 13 | 3919.6 | 1 | Acidothermus cellulolyticus 11B | ||
| 6 | F (Y) | DNA non-target strand (5'-D(P*AP*GP*A)-3') | polymer | 3 | 910.7 | 1 | Acidothermus cellulolyticus 11B | ||
| 7 | G, H, I, J, K... (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 10 | Chemie (MG) | |||
| 8 | Q, R, S, T, U (A, B, X, T, D) | water | water | 18.0 | 300 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 177956.9 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 243.1 | |
| All* | Total formula weight | 178200.0 |
