8CWT
Complex structure of WhiB3 and the SigmaAr4-RNAP Beta flap tip chimera in space group P43212
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C, E | Redox- and pH-responsive transcriptional regulator WhiB3 | polymer | 90 | 10268.6 | 3 | UniProt (P9WF41) Pfam (PF02467) In PDB | Mycobacterium tuberculosis H37Rv | |
2 | B, D, F | RNA polymerase sigma factor SigA,DNA-directed RNA polymerase subunit beta | polymer | 112 | 12650.2 | 3 | UniProt (P9WGI1) UniProt (P9WGY9) Pfam (PF04545) In PDB | Mycobacterium tuberculosis H37Rv | Sigma-A,RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
3 | A, E, C | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 3 | Chemie (SF4) | |||
4 | D, A, E, B, F... | SULFATE ION | non-polymer | 96.1 | 25 | Chemie (SO4) | |||
5 | B, F, D | NICKEL (II) ION | non-polymer | 58.7 | 7 | Chemie (NI) | |||
6 | D, F, B | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 3 | Chemie (TRS) | |||
7 | water | water | 18.0 | 522 | Chemie (HOH) |
Sequence modifications
B, D, F: 446 - 528 (UniProt: P9WGI1)
B, D, F: 535 - 549 (UniProt: P9WGY9)
PDB | External Database | Details |
---|---|---|
Met 438 | - | initiating methionine |
Ala 439 | - | expression tag |
His 440 | - | expression tag |
His 441 | - | expression tag |
His 442 | - | expression tag |
His 443 | - | expression tag |
His 444 | - | expression tag |
His 445 | - | expression tag |
Gly 529 | - | linker |
Ser 530 | - | linker |
Ser 531 | - | linker |
Gly 532 | - | linker |
Ser 533 | - | linker |
Gly 534 | - | linker |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 68756.6 | |
Non-Polymers* | Number of molecules | 38 |
Total formula weight | 4233.8 | |
All* | Total formula weight | 72990.4 |