8CVH
Structure of L289F Hyoscyamine 6-beta Hydroxylase in complex with vanadyl, succinate, and 6-OH-hyoscyamine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Hyoscyamine 6-beta-hydroxylase | polymer | 385 | 42667.0 | 1 | UniProt (Q9XJ43) Pfam (PF14226) Pfam (PF03171) | Atropa belladonna (belladonna) | |
| 2 | B, C, D, H, K (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 5 | Chemie (EDO) | |||
| 3 | E, F, G, L, M... (A) | FORMIC ACID | non-polymer | 46.0 | 6 | Chemie (FMT) | |||
| 4 | I (A) | SUCCINIC ACID | non-polymer | 118.1 | 1 | Chemie (SIN) | |||
| 5 | J (A) | (1R,3S,5R,6S)-6-hydroxy-8-methyl-8-azabicyclo[3.2.1]octan-3-yl (2S)-3-hydroxy-2-phenylpropanoate | non-polymer | 305.4 | 1 | Chemie (OVR) | |||
| 6 | O (A) | VANADIUM ION | non-polymer | 50.9 | 1 | Chemie (V) | |||
| 7 | P, Q (A) | STRONTIUM ION | non-polymer | 87.6 | 2 | Chemie (SR) | |||
| 8 | R (A) | water | water | 18.0 | 101 | Chemie (HOH) |
Sequence modifications
A: 1 - 343 (UniProt: Q9XJ43)
| PDB | External Database | Details |
|---|---|---|
| Phe 289 | Leu 289 | engineered mutation |
| Gly 344 | - | expression tag |
| Gly 345 | - | expression tag |
| Lys 346 | - | expression tag |
| Gly 347 | - | expression tag |
| Pro 348 | - | expression tag |
| Gly 349 | - | expression tag |
| Asp 350 | - | expression tag |
| Gly 351 | - | expression tag |
| Pro 352 | - | expression tag |
| Gly 353 | - | expression tag |
| Gly 354 | - | expression tag |
| Ser 355 | - | expression tag |
| Gly 356 | - | expression tag |
| Gly 357 | - | expression tag |
| Pro 358 | - | expression tag |
| Arg 359 | - | expression tag |
| Gly 360 | - | expression tag |
| Gly 361 | - | expression tag |
| Glu 362 | - | expression tag |
| Asn 363 | - | expression tag |
| Leu 364 | - | expression tag |
| Tyr 365 | - | expression tag |
| Phe 366 | - | expression tag |
| Gln 367 | - | expression tag |
| Ser 368 | - | expression tag |
| Gly 369 | - | expression tag |
| His 370 | - | expression tag |
| His 371 | - | expression tag |
| His 372 | - | expression tag |
| His 373 | - | expression tag |
| His 374 | - | expression tag |
| His 375 | - | expression tag |
| His 376 | - | expression tag |
| His 377 | - | expression tag |
| Gly 378 | - | expression tag |
| Gly 379 | - | expression tag |
| Arg 380 | - | expression tag |
| Gly 381 | - | expression tag |
| Gly 382 | - | expression tag |
| Ala 383 | - | expression tag |
| Ala 384 | - | expression tag |
| Ala 385 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 42667.0 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 1236.1 | |
| All* | Total formula weight | 43903.1 |
