8CVH
Structure of L289F Hyoscyamine 6-beta Hydroxylase in complex with vanadyl, succinate, and 6-OH-hyoscyamine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Hyoscyamine 6-beta-hydroxylase | polymer | 385 | 42667.0 | 1 | UniProt (Q9XJ43) | Atropa belladonna (belladonna) | |
| 2 | B, C, D, H, K (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 5 | Chemie (EDO) | |||
| 3 | E, F, G, L, M... (A) | FORMIC ACID | non-polymer | 46.0 | 6 | Chemie (FMT) PubChem (284) | |||
| 4 | I (A) | SUCCINIC ACID | non-polymer | 118.1 | 1 | Chemie (SIN) PubChem (1110) | |||
| 5 | J (A) | (1R,3S,5R,6S)-6-hydroxy-8-methyl-8-azabicyclo[3.2.1]octan-3-yl (2S)-3-hydroxy-2-phenylpropanoate | non-polymer | 305.4 | 1 | Chemie (OVR) PubChem (2198) PubChem (22525014) PubChem (177401097) PubChem (25028101) PubChem (40542655) PubChem (64704) PubChem (50930264) PubChem (51026956) PubChem (442993) PubChem (71711121) PubChem (71761052) PubChem (73012293) PubChem (73440795) PubChem (76965511) PubChem (906762) PubChem (929216) PubChem (1403492) PubChem (183088) PubChem (439976) PubChem (71587892) PubChem (86308943) PubChem (91746610) PubChem (5459777) PubChem (6918612) PubChem (7040178) PubChem (5701991) PubChem (129846196) PubChem (6571525) PubChem (6710677) PubChem (98072234) PubChem (98145194) PubChem (100817658) PubChem (101995422) PubChem (7002013) PubChem (10017971) PubChem (124717432) PubChem (124717433) PubChem (154831942) PubChem (168313405) PubChem (126969949) PubChem (129628892) PubChem (131954667) PubChem (134693125) PubChem (139033153) PubChem (162981523) PubChem (162981524) PubChem (163355666) PubChem (168011994) | |||
| 6 | O (A) | VANADIUM ION | non-polymer | 50.9 | 1 | Chemie (V) | |||
| 7 | P, Q (A) | STRONTIUM ION | non-polymer | 87.6 | 2 | Chemie (SR) PubChem (104798) | |||
| 8 | R (A) | water | water | 18.0 | 101 | Chemie (HOH) |
Sequence modifications
A: 1 - 343 (UniProt: Q9XJ43)
| PDB | External Database | Details |
|---|---|---|
| Phe 289 | Leu 289 | engineered mutation |
| Gly 344 | - | expression tag |
| Gly 345 | - | expression tag |
| Lys 346 | - | expression tag |
| Gly 347 | - | expression tag |
| Pro 348 | - | expression tag |
| Gly 349 | - | expression tag |
| Asp 350 | - | expression tag |
| Gly 351 | - | expression tag |
| Pro 352 | - | expression tag |
| Gly 353 | - | expression tag |
| Gly 354 | - | expression tag |
| Ser 355 | - | expression tag |
| Gly 356 | - | expression tag |
| Gly 357 | - | expression tag |
| Pro 358 | - | expression tag |
| Arg 359 | - | expression tag |
| Gly 360 | - | expression tag |
| Gly 361 | - | expression tag |
| Glu 362 | - | expression tag |
| Asn 363 | - | expression tag |
| Leu 364 | - | expression tag |
| Tyr 365 | - | expression tag |
| Phe 366 | - | expression tag |
| Gln 367 | - | expression tag |
| Ser 368 | - | expression tag |
| Gly 369 | - | expression tag |
| His 370 | - | expression tag |
| His 371 | - | expression tag |
| His 372 | - | expression tag |
| His 373 | - | expression tag |
| His 374 | - | expression tag |
| His 375 | - | expression tag |
| His 376 | - | expression tag |
| His 377 | - | expression tag |
| Gly 378 | - | expression tag |
| Gly 379 | - | expression tag |
| Arg 380 | - | expression tag |
| Gly 381 | - | expression tag |
| Gly 382 | - | expression tag |
| Ala 383 | - | expression tag |
| Ala 384 | - | expression tag |
| Ala 385 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 42667.0 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 1236.1 | |
| All* | Total formula weight | 43903.1 |
