8CTQ

Crystal structure of engineered phospholipase D mutant superPLD 2-48

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Phospholipase D	polymer	513	54796.2	1	UniProt (P84147) Pfam (PF13091)	Streptomyces sp. PMF
2	B (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
3	C (A)	water	water		18.0	284	Chemie (HOH)

Sequence modifications

A: 1 - 506 (UniProt: P84147)

PDB	External Database	Details
Gly -6	-	expression tag
Pro -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Arg 57	Lys 57	engineered mutation
Val 59	Ala 59	engineered mutation
Arg 109	Lys 109	engineered mutation
Ala 245	Pro 245	engineered mutation
Ile 264	Val 264	engineered mutation
Ser 328	Gly 328	engineered mutation
Val 381	Gly 381	engineered mutation
Ser 406	Gly 406	engineered mutation
Asp 429	Gly 429	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	54796.2
Non-Polymers*	Number of molecules	1
	Total formula weight	95.0
All*	Total formula weight	54891.1

*Water molecules are not included.