8CRN

Streptavidin S112Y Co-TAML artificial metalloenzyme

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Streptavidin	polymer	158	16513.9	4	UniProt (P22629) Pfam (PF01382)	Streptomyces avidinii
2	E, G, I, J (A, B, C, D)	Co-linked Tetra-amido macrocyclic ligand	non-polymer		670.6	4	Chemie (VJL)
3	F (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
4	H (B)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	K, L, M, N (A, B, C, D)	water	water		18.0	126	Chemie (HOH)

Sequence modifications

A, B, C, D: 15 - 159 (UniProt: P22629)

PDB	External Database	Details
Ala 2	-	expression tag
Ser 3	-	expression tag
Met 4	-	expression tag
Thr 5	-	expression tag
Gly 6	-	expression tag
Gly 7	-	expression tag
Gln 8	-	expression tag
Gln 9	-	expression tag
Met 10	-	expression tag
Gly 11	-	expression tag
Arg 12	-	expression tag
Asp 13	-	expression tag
Gln 14	-	expression tag
Tyr 112	Ser 136	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	66055.7
Non-Polymers*	Number of molecules	6
	Total formula weight	2801.6
All*	Total formula weight	68857.4

*Water molecules are not included.