8CRD

Aspergillus niger ferulic acid decarboxylase (Fdc) T40C-S315C (DB1) variant in complex with prenylated flavin hydroxylated at the C1 prime position

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ferulic acid decarboxylase 1	polymer	508	56354.0	1	UniProt (A2QHE5) Pfam (PF20695) Pfam (PF01977) Pfam (PF20696) In PDB	Aspergillus niger CBS 513.88	Phenacrylate decarboxylase
2	A	hydroxylated prenyl-FMN	non-polymer		542.5	1	Chemie (BYN)
3	A	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
4	A	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
5	A	THIOCYANATE ION	non-polymer		58.1	4	Chemie (SCN)
6		water	water		18.0	552	Chemie (HOH)

Sequence modifications

A: 1 - 500 (UniProt: A2QHE5)

PDB	External Database	Details
Cys 40	Thr 40	engineered mutation
Cys 315	Ser 315	engineered mutation
Leu 501	-	expression tag
Glu 502	-	expression tag
His 503	-	expression tag
His 504	-	expression tag
His 505	-	expression tag
His 506	-	expression tag
His 507	-	expression tag
His 508	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	56354.0
Non-Polymers*	Number of molecules	8
	Total formula weight	907.9
All*	Total formula weight	57262.0

*Water molecules are not included.