8CQG

Crystal Structure of a Chimeric Alpha-Amylase from Pseudoalteromonas Haloplanktis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Alpha-amylase	polymer	450	49230.0	1	UniProt (P29957)	Pseudoalteromonas haloplanktis	1,4-alpha-D-glucan glucanohydrolase
2	B, C, D, E, F... (A)	1,2-ETHANEDIOL	non-polymer		62.1	11	Chemie (EDO)
3	M, N (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL) PubChem (312)
4	O (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA) PubChem (271)
5	P (A)	water	water		18.0	396	Chemie (HOH)

Sequence modifications

A: 1 - 208 (UniProt: P29957)

PDB	External Database	Details
Gly 0	-	expression tag
Val 77	Ala 101	engineered mutation
Leu 204	Gln 228	engineered mutation

A: 212 - 269 (UniProt: P29957)

PDB	External Database	Details
Ile 209	-	linker
Lys 210	-	linker
Ser 211	-	linker
Gly 226	Ser 250	engineered mutation
Ala 227	Thr 251	engineered mutation
Lys 228	Glu 252	engineered mutation
Thr 231	Asn 255	engineered mutation
Val 232	Thr 256	engineered mutation

A: 278 - 447 (UniProt: P29957)

PDB	External Database	Details
Ala 269	-	linker
Gly 269	-	linker
Gly 272	-	linker
Ser 273	-	linker
Ser 274	-	linker
Ile 275	-	linker
Leu 276	-	linker
Thr 277	-	linker
Arg 300	Lys 324	engineered mutation
Asn 310	Asp 334	engineered mutation
Asp 311	Thr 335	engineered mutation
Trp 312	Asp 336	engineered mutation
Leu 448	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	49230.0
Non-Polymers*	Number of molecules	14
	Total formula weight	793.7
All*	Total formula weight	50023.8

*Water molecules are not included.