8CM6

W-formate dehydrogenase C872A from Desulfovibrio vulgaris - with Formamide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Formate dehydrogenase, alpha subunit, selenocysteine-containing	polymer	1013	112405.0	2	UniProt (Q72EJ1) Pfam (PF04879) Pfam (PF00384) Pfam (PF01568) In PDB	Desulfovibrio vulgaris str. Hildenborough
2	B, D	Formate dehydrogenase, beta subunit, putative	polymer	215	23989.5	2	UniProt (Q72EJ0) Pfam (PF13247) In PDB	Desulfovibrio vulgaris str. Hildenborough
3	A, C	HYDROSULFURIC ACID	non-polymer		34.1	2	Chemie (H2S)
4	A, C	TUNGSTEN ION	non-polymer		183.8	2	Chemie (W)
5	A, C	2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE GUANOSINE DINUCLEOTIDE	non-polymer		740.6	4	Chemie (MGD)
6	B, A, C, D	IRON/SULFUR CLUSTER	non-polymer		351.6	8	Chemie (SF4)
7	B, A, D	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
8	A, C	GLYCEROL	non-polymer		92.1	14	Chemie (GOL)
9	B, C, A	TETRAETHYLENE GLYCOL	non-polymer		194.2	4	Chemie (PG4)
10	C, A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
11	B, C, A	FORMAMIDE	non-polymer		45.0	7	Chemie (ARF)
12	B, D	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
13		water	water		18.0	1718	Chemie (HOH)

Sequence modifications

A, C: 1 - 1005 (UniProt: Q72EJ1)

PDB	External Database	Details
Ala 872	Cys 872	engineered mutation
Trp 1006	-	expression tag
Ser 1007	-	expression tag
His 1008	-	expression tag
Pro 1009	-	expression tag
Gln 1010	-	expression tag
Phe 1011	-	expression tag
Glu 1012	-	expression tag
Lys 1013	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	272788.9
Non-Polymers*	Number of molecules	48
	Total formula weight	9035.4
All*	Total formula weight	281824.4

*Water molecules are not included.