8CM5

W-formate dehydrogenase C872A from Desulfovibrio vulgaris

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, K, R)	Formate dehydrogenase, alpha subunit, selenocysteine-containing	polymer	1013	112405.0	4	UniProt (Q72EJ1) Pfam (PF04879) Pfam (PF00384) Pfam (PF01568)	Desulfovibrio vulgaris str. Hildenborough
2	B, D, F, H (B, D, L, S)	Formate dehydrogenase, beta subunit, putative	polymer	215	23989.5	4	UniProt (Q72EJ0) Pfam (PF13247)	Desulfovibrio vulgaris str. Hildenborough
3	GA, HA, I, R, RA... (L, A, C, S, D)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	6	Chemie (PEG)
4	BA, J, LA, S (K, A, R, C)	HYDROSULFURIC ACID	non-polymer		34.1	4	Chemie (H2S)
5	CA, K, MA, T (K, A, R, C)	TUNGSTEN ION	non-polymer		183.8	4	Chemie (W)
6	DA, EA, L, M, NA... (K, A, R, C)	2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE GUANOSINE DINUCLEOTIDE	non-polymer		740.6	8	Chemie (MGD)
7	AA, FA, IA, JA, KA... (D, K, L, A, B...)	IRON/SULFUR CLUSTER	non-polymer		351.6	16	Chemie (SF4)
8	QA (S)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
9	AB, BB, CB, VA, WA... (L, R, S, A, B...)	water	water		18.0	963	Chemie (HOH)

Sequence modifications

A, C, K, R: 1 - 1005 (UniProt: Q72EJ1)

PDB	External Database	Details
Ala 872	Cys 872	engineered mutation
Trp 1006	-	expression tag
Ser 1007	-	expression tag
His 1008	-	expression tag
Pro 1009	-	expression tag
Gln 1010	-	expression tag
Phe 1011	-	expression tag
Glu 1012	-	expression tag
Lys 1013	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	545577.9
Non-Polymers*	Number of molecules	39
	Total formula weight	13151.2
All*	Total formula weight	558729.1

*Water molecules are not included.