8CJA
A225L/F231A variant of the CODH/ACS complex of C. hydrogenoformans
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Carbon monoxide dehydrogenase | polymer | 669 | 73096.0 | 1 | UniProt (A0A1L8D0M5) | Carboxydothermus hydrogenoformans Z-2901 | |
| 2 | B (B) | CO-methylating acetyl-CoA synthase | polymer | 731 | 82291.4 | 1 | UniProt (Q3ACS4) Pfam (PF18537) Pfam (PF03598) Pfam (PF19436) | Carboxydothermus hydrogenoformans Z-2901 | |
| 3 | C, D (A) | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 2 | Chemie (MPD) PubChem (5288834) | |||
| 4 | E (A) | Fe(3)-Ni(1)-S(4) cluster | non-polymer | 410.3 | 1 | Chemie (RQM) | |||
| 5 | F, I, L (A, B) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 3 | Chemie (SF4) | |||
| 6 | G, H (A) | HYDROXIDE ION | non-polymer | 17.0 | 2 | Chemie (OH) PubChem (961) | |||
| 7 | J (B) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) PubChem (175) | |||
| 8 | K, M (B) | NICKEL (II) ION | non-polymer | 58.7 | 2 | Chemie (NI) PubChem (934) | |||
| 9 | N, O (A, B) | water | water | 18.0 | 743 | Chemie (HOH) |
Sequence modifications
A: 2 - 670 (UniProt: A0A1L8D0M5)
B: 5 - 732 (UniProt: Q3ACS4)
| PDB | External Database | Details |
|---|---|---|
| Asp 17 | Glu 17 | conflict |
| Ile 29 | Thr 29 | conflict |
| Gln 73 | Met 73 | conflict |
| Ala 120 | Thr 120 | conflict |
| Thr 153 | Ile 153 | conflict |
| Met 159 | Leu 159 | conflict |
| Glu 199 | Asp 199 | conflict |
| Ser 205 | Ala 205 | conflict |
| Ile 220 | Met 220 | conflict |
| Ala 231 | Phe 231 | engineered mutation |
| Ile 389 | Val 389 | conflict |
| Leu 393 | Phe 393 | conflict |
| Thr 494 | Ala 494 | conflict |
| Thr 602 | Ser 602 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Leu 225 | Ala 225 | engineered mutation |
| Arg 733 | - | expression tag |
| Ser 734 | - | expression tag |
| His 735 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 155387.4 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1912.0 | |
| All* | Total formula weight | 157299.4 |
