8CIA

Crystal structure of the kelch domain of human KLHL20

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, D	Kelch-like protein 20	polymer	307	33321.4	3	UniProt (Q9Y2M5) Pfam (PF01344) In PDB	Homo sapiens (human)	Kelch-like ECT2-interacting protein,Kelch-like protein X
2	B	Kelch like family member 20	polymer	307	33321.5	1	UniProt (A0A673T7L2) Pfam (PF01344) UniProt (by SIFTS) (Q9Y2M5) In PDB	Homo sapiens (human)
3	E	Kelch like family member 20	polymer	307	33322.4	1	UniProt (A0A452F0N4) Pfam (PF01344) UniProt (by SIFTS) (Q9Y2M5) In PDB	Homo sapiens (human)

Sequence modifications

A, C, D: 299 - 605 (UniProt: Q9Y2M5)

PDB	External Database	Details
Gly 299	Glu 299	conflict
His 300	Arg 300	conflict
Met 301	Pro 301	conflict
Ser 302	Leu 302	conflict
Ala 316	Cys 316	engineered mutation
Ala 327	Cys 327	engineered mutation
Ala 402	Cys 402	engineered mutation
Ala 426	Cys 426	engineered mutation
Ala 604	Cys 604	engineered mutation

B: 303 - 605 (UniProt: A0A673T7L2)

PDB	External Database	Details
Gly 299	-	expression tag
His 300	-	expression tag
Met 301	-	expression tag
Ser 302	-	expression tag
Ala 316	Cys 316	conflict
Ala 327	Cys 327	conflict
Ala 402	Cys 402	conflict
Ala 426	Cys 426	conflict
Lys 437	Glu 437	conflict
Ala 604	Cys 604	conflict

E: 303 - 605 (UniProt: A0A452F0N4)

PDB	External Database	Details
Gly 299	-	expression tag
His 300	-	expression tag
Met 301	-	expression tag
Ser 302	-	expression tag
Ala 316	Cys 315	conflict
Ala 327	Cys 326	conflict
Ala 402	Cys 401	conflict
Ala 426	Cys 425	conflict
Glu 483	Gln 482	conflict
Ala 604	Cys 603	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	166608.1
All*	Total formula weight	166608.1

*Water molecules are not included.