8CE4

Human alpha7 nicotinic receptor in complex with the E3 nanobody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Neuronal acetylcholine receptor subunit alpha-7	polymer	388	44050.9	5	UniProt (P36544) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)
2	F, G, H, I, J (F, G, H, I, J)	Nanobody E3	polymer	149	15740.2	5		Vicugna pacos
3	K, L, M, N, O (P, Q, R, S, T)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	5	In PDB GlyTouCan (G42666HT)
4	P, Q, R, S, T... (A, B, C, D, E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	10	Chemie (NAG)

Sequence modifications

A, B, C, D, E: 1 - 324 (UniProt: P36544)
A, B, C, D, E: 328 - 375 (UniProt: P36544)

PDB	External Database	Details
Asp 325	-	linker
Gly 326	-	linker
Val 327	-	linker
Ser 376	-	expression tag
Ala 377	-	expression tag
Gly 378	-	expression tag
Leu 379	-	expression tag
Thr 380	-	expression tag
Glu 381	-	expression tag
Thr 382	-	expression tag
Ser 383	-	expression tag
Gln 384	-	expression tag
Val 385	-	expression tag
Ala 386	-	expression tag
Pro 387	-	expression tag
Ala 388	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	298955.7
Branched	Number of molecules	5
	Total formula weight	2122.0
Non-Polymers*	Number of molecules	10
	Total formula weight	2212.1
All*	Total formula weight	303289.8

*Water molecules are not included.