8CBC
Crystal structure of Thermothelomyces thermophila GH30 (double mutant EE) in complex with xylotriose.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | GH30 family xylanase | polymer | 455 | 48482.4 | 2 | UniProt (G2Q1N4) Pfam (PF14587) Pfam (PF17189) | Thermothelomyces | |
| 2 | C (C) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1559.4 | 1 | In PDB GlyTouCan (G83161QT) | |||
| 3 | D (D) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 4 | E, F (E, F) | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-D-xylose | branched | 414.4 | 2 | In PDB | |||
| 5 | G, H (G, H) | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose | branched | 282.2 | 2 | In PDB BIRD (PRD_900117) GlyTouCan (G87728WL) | |||
| 6 | I, J, K, L (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
| 7 | M (B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 8 | N, O (A, B) | water | water | 18.0 | 831 | Chemie (HOH) |
Sequence modifications
A, B: 7 - 460 (UniProt: G2Q1N4)
| PDB | External Database | Details |
|---|---|---|
| Ala 188 | Glu 205 | engineered mutation |
| Ala 278 | Glu 295 | engineered mutation |
| Leu 461 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 96964.9 | |
| Branched | Number of molecules | 6 |
| Total formula weight | 3377.0 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 354.4 | |
| All* | Total formula weight | 100696.3 |
