8C8H

Cryo EM structure of the vaccinia complete RNA polymerase complex lacking the capping enzyme

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	DNA-directed RNA polymerase 133 kDa polypeptide	polymer	1164	133526.9	1	UniProt (Q76ZP7) Pfam (PF04560) Pfam (PF12415) Pfam (PF04565) Pfam (PF04567) Pfam (PF00562)	Vaccinia virus GLV-1h68
2	B (E)	DNA-directed RNA polymerase 22 kDa subunit	polymer	185	21365.7	1	UniProt (P68609) Pfam (PF05273)	Vaccinia virus GLV-1h68
3	C (F)	DNA-directed RNA polymerase 19 kDa subunit	polymer	164	19020.1	1	UniProt (P68611) Pfam (PF05320)	Vaccinia virus GLV-1h68
4	D (G)	DNA-directed RNA polymerase 18 kDa subunit	polymer	161	17917.2	1	UniProt (P04310) Pfam (PF03293)	Vaccinia virus GLV-1h68
5	E (I)	RNA polymerase-associated transcription-specificity factor RAP94	polymer	795	93667.6	1	UniProt (P68438) Pfam (PF03294)	Vaccinia virus GLV-1h68	Protein H4,RPO-associated protein of 94 kDa
6	F (J)	DNA-directed RNA polymerase 7 kDa subunit	polymer	63	7299.7	1	UniProt (P68317) Pfam (PF05864)	Vaccinia virus GLV-1h68
7	G, K (R, Q)	Core protein E11	polymer	129	14914.1	2	UniProt (P68448) Pfam (PF06138)	Vaccinia virus GLV-1h68
8	H (K)	Early transcription factor 82 kDa subunit	polymer	710	82398.9	1	UniProt (P20636) Pfam (PF04441)	Vaccinia virus GLV-1h68	ETF large subunit,VETF A7 subunit,Vaccinia virus early transcription factor large subunit,VETF large subunit
9	I (U)	chr17.trna16-GlnTTG	polymer	72	23108.7	1		Vaccinia virus GLV-1h68
10	J (A)	DNA-directed RNA polymerase 147 kDa polypeptide	polymer	1286	146995.7	1	UniProt (P07392) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Vaccinia virus GLV-1h68
11	L (Y)	Nucleoside triphosphatase I	polymer	631	72465.3	1	UniProt (P05807) Pfam (PF00176) Pfam (PF00271) Pfam (PF08469)	Vaccinia virus GLV-1h68	Factor X,NPH-I,Nucleoside triphosphate phosphohydrolase I,NPH I
12	M (C)	DNA-directed RNA polymerase 35 kDa subunit	polymer	305	35430.7	1	UniProt (P24757) Pfam (PF03396)	Vaccinia virus GLV-1h68
13	N (S)	DNA-directed RNA polymerase 30 kDa polypeptide	polymer	259	30074.3	1	UniProt (P21603) Pfam (PF12410) Pfam (PF01096)	Vaccinia virus GLV-1h68
14	O, P, R, S (B, I, A)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
15	Q (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
16	AA, BA, CA, T, U... (A, Y, C, B, E...)	water	water		18.0	61	Chemie (HOH)

Sequence modifications

B: 1 - 1164 (UniProt: Q76ZP7)

PDB	External Database	Details
Asn 6	Asp 6	conflict
Phe 343	Tyr 343	variant

G: 1 - 161 (UniProt: P04310)

PDB	External Database	Details
Ser 11	Pro 11	variant

I: 1 - 795 (UniProt: P68438)

PDB	External Database	Details
Cys 623	Tyr 623	variant
Val 624	Ile 624	variant

K: 1 - 707 (UniProt: P20636)

PDB	External Database	Details
Arg 401	Lys 401	variant

A: 1 - 1286 (UniProt: P07392)

PDB	External Database	Details
Thr 258	Ser 258	variant
Asn 1006	Asp 1006	variant
Ile 1007	His 1007	variant
Thr 1008	Ser 1008	variant
-	Phe 1010	deletion
Arg 1010	Glu 1011	variant
Glu 1012	Lys 1013	variant
Thr 1013	Gln 1014	variant
Lys 1015	Arg 1016	variant
Arg 1024	Lys 1025	variant
Asp 1113	Cys 1114	variant

Y: 1 - 631 (UniProt: P05807)

PDB	External Database	Details
Lys 342	Arg 342	variant
Lys 347	Gly 347	variant
Lys 568	Asn 568	variant

C: 1 - 305 (UniProt: P24757)

PDB	External Database	Details
Phe 212	Leu 212	variant

S: 1 - 259 (UniProt: P21603)

PDB	External Database	Details
Ala 20	Thr 20	variant

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	713099.0
Non-Polymers*	Number of molecules	5
	Total formula weight	285.9
All*	Total formula weight	713384.9

*Water molecules are not included.