8C83
Cryo-EM structure of in vitro reconstituted Otu2-bound Ub-40S complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (x) | OTU domain-containing protein 2 | polymer | 307 | 36157.0 | 1 | UniProt (P38747) Pfam (PF02338) | Saccharomyces cerevisiae (baker's yeast) | |
| 2 | B (y) | Ubiquitin | polymer | 76 | 8576.8 | 1 | UniProt (P05759) Pfam (PF00240) | Saccharomyces cerevisiae (baker's yeast) | |
| 3 | C (2) | 18S ribosomal RNA | polymer | 1800 | 579871.1 | 1 | Saccharomyces cerevisiae (baker's yeast) | ||
| 4 | D (P) | 40S ribosomal protein S0-A | polymer | 252 | 28051.3 | 1 | UniProt (P32905) Pfam (PF00318) | Saccharomyces cerevisiae (baker's yeast) | Nucleic acid-binding protein NAB1A,Small ribosomal subunit protein uS2-A |
| 5 | E (R) | 40S ribosomal protein S2 | polymer | 254 | 27490.8 | 1 | UniProt (P25443) Pfam (PF00333) Pfam (PF03719) | Saccharomyces cerevisiae (baker's yeast) | Omnipotent suppressor protein SUP44,RP12,S4,Small ribosomal subunit protein uS5,YS5 |
| 6 | F (S) | 40S ribosomal protein S4-A | polymer | 261 | 29469.3 | 1 | UniProt (P0CX35) Pfam (PF08071) Pfam (PF01479) Pfam (PF00900) Pfam (PF00467) Pfam (PF16121) | Saccharomyces cerevisiae (baker's yeast) | RP5,S7,Small ribosomal subunit protein eS4-A,YS6 |
| 7 | G (T) | 40S ribosomal protein S6-A | polymer | 236 | 27054.5 | 1 | UniProt (P0CX37) Pfam (PF01092) | Saccharomyces cerevisiae (baker's yeast) | RP9,S10,Small ribosomal subunit protein eS6-A,YS4 |
| 8 | H (U) | 40S ribosomal protein S7-A | polymer | 190 | 21658.2 | 1 | UniProt (P26786) Pfam (PF01251) | Saccharomyces cerevisiae (baker's yeast) | RP30,RP40,Small ribosomal subunit protein eS7-A |
| 9 | I (V) | 40S ribosomal protein S8-B | polymer | 200 | 22537.8 | 1 | UniProt (P0CX40) Pfam (PF01201) | Saccharomyces cerevisiae (baker's yeast) | RP19,S14,Small ribosomal subunit protein eS8-B,YS9 |
| 10 | J (W) | 40S ribosomal protein S9-A | polymer | 197 | 22487.9 | 1 | UniProt (O13516) Pfam (PF00163) Pfam (PF01479) | Saccharomyces cerevisiae (baker's yeast) | RP21,S13,Small ribosomal subunit protein uS4-A,YP28,YS11 |
| 11 | K (X) | 40S ribosomal protein S11-A | polymer | 156 | 17785.9 | 1 | UniProt (P0CX47) Pfam (PF16205) Pfam (PF00366) | Saccharomyces cerevisiae (baker's yeast) | RP41,S18,Small ribosomal subunit protein uS17-A,YS12 |
| 12 | L (Y) | 40S ribosomal protein S13 | polymer | 151 | 17059.9 | 1 | UniProt (P05756) Pfam (PF08069) Pfam (PF00312) | Saccharomyces cerevisiae (baker's yeast) | S27a,Small ribosomal subunit protein uS15,YS15 |
| 13 | M (a) | 40S ribosomal protein S21-A | polymer | 87 | 9758.8 | 1 | UniProt (P0C0V8) Pfam (PF01249) | Saccharomyces cerevisiae (baker's yeast) | S26,Small ribosomal subunit protein eS21-A,YS25 |
| 14 | N (b) | 40S ribosomal protein S22-A | polymer | 130 | 14650.1 | 1 | UniProt (P0C0W1) Pfam (PF00410) | Saccharomyces cerevisiae (baker's yeast) | RP50,S24,Small ribosomal subunit protein uS8-A,YP58,YS22 |
| 15 | O (c) | 40S ribosomal protein S23-A | polymer | 145 | 16073.9 | 1 | UniProt (P0CX29) Pfam (PF00164) | Saccharomyces cerevisiae (baker's yeast) | RP37,S28,Small ribosomal subunit protein uS12-A,YS14 |
| 16 | P (d) | 40S ribosomal protein S24-A | polymer | 135 | 15362.8 | 1 | UniProt (P0CX31) Pfam (PF01282) | Saccharomyces cerevisiae (baker's yeast) | RP50,Small ribosomal subunit protein eS24-A |
| 17 | Q (f) | 40S ribosomal protein S27-A | polymer | 82 | 8893.4 | 1 | UniProt (P35997) Pfam (PF01667) | Saccharomyces cerevisiae (baker's yeast) | RP61,Small ribosomal subunit protein eS27-A,YS20 |
| 18 | R (g) | 40S ribosomal protein S30-A | polymer | 63 | 7137.5 | 1 | UniProt (P0CX33) Pfam (PF04758) | Saccharomyces cerevisiae (baker's yeast) | Small ribosomal subunit protein eS30-A |
Sequence modifications
x: 1 - 307 (UniProt: P38747)
y: 1 - 76 (UniProt: P05759)
| PDB | External Database | Details |
|---|---|---|
| Ser 178 | Cys 178 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Pro 19 | Ser 19 | conflict |
| Glu 24 | Asp 24 | conflict |
| Ala 28 | Ser 28 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 18 |
| Total formula weight | 910077.2 | |
| All* | Total formula weight | 910077.2 |
