8C83

Cryo-EM structure of in vitro reconstituted Otu2-bound Ub-40S complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	x	OTU domain-containing protein 2	polymer	307	36157.0	1	UniProt (P38747) Pfam (PF02338) In PDB	Saccharomyces cerevisiae (baker's yeast)
2	y	Ubiquitin	polymer	76	8576.8	1	UniProt (P05759) Pfam (PF00240) In PDB	Saccharomyces cerevisiae (baker's yeast)
3	2	18S ribosomal RNA	polymer	1800	579871.1	1		Saccharomyces cerevisiae (baker's yeast)
4	P	40S ribosomal protein S0-A	polymer	252	28051.3	1	UniProt (P32905) Pfam (PF00318) In PDB	Saccharomyces cerevisiae (baker's yeast)	Nucleic acid-binding protein NAB1A,Small ribosomal subunit protein uS2-A
5	R	40S ribosomal protein S2	polymer	254	27490.8	1	UniProt (P25443) Pfam (PF00333) Pfam (PF03719) In PDB	Saccharomyces cerevisiae (baker's yeast)	Omnipotent suppressor protein SUP44,RP12,S4,Small ribosomal subunit protein uS5,YS5
6	S	40S ribosomal protein S4-A	polymer	261	29469.3	1	UniProt (P0CX35) Pfam (PF08071) Pfam (PF01479) Pfam (PF00900) Pfam (PF00467) Pfam (PF16121) In PDB	Saccharomyces cerevisiae (baker's yeast)	RP5,S7,Small ribosomal subunit protein eS4-A,YS6
7	T	40S ribosomal protein S6-A	polymer	236	27054.5	1	UniProt (P0CX37) Pfam (PF01092) In PDB	Saccharomyces cerevisiae (baker's yeast)	RP9,S10,Small ribosomal subunit protein eS6-A,YS4
8	U	40S ribosomal protein S7-A	polymer	190	21658.2	1	UniProt (P26786) Pfam (PF01251) In PDB	Saccharomyces cerevisiae (baker's yeast)	RP30,RP40,Small ribosomal subunit protein eS7-A
9	V	40S ribosomal protein S8-B	polymer	200	22537.8	1	UniProt (P0CX40) Pfam (PF01201) In PDB	Saccharomyces cerevisiae (baker's yeast)	RP19,S14,Small ribosomal subunit protein eS8-B,YS9
10	W	40S ribosomal protein S9-A	polymer	197	22487.9	1	UniProt (O13516) Pfam (PF00163) Pfam (PF01479) In PDB	Saccharomyces cerevisiae (baker's yeast)	RP21,S13,Small ribosomal subunit protein uS4-A,YP28,YS11
11	X	40S ribosomal protein S11-A	polymer	156	17785.9	1	UniProt (P0CX47) Pfam (PF16205) Pfam (PF00366) In PDB	Saccharomyces cerevisiae (baker's yeast)	RP41,S18,Small ribosomal subunit protein uS17-A,YS12
12	Y	40S ribosomal protein S13	polymer	151	17059.9	1	UniProt (P05756) Pfam (PF08069) Pfam (PF00312) In PDB	Saccharomyces cerevisiae (baker's yeast)	S27a,Small ribosomal subunit protein uS15,YS15
13	a	40S ribosomal protein S21-A	polymer	87	9758.8	1	UniProt (P0C0V8) Pfam (PF01249) In PDB	Saccharomyces cerevisiae (baker's yeast)	S26,Small ribosomal subunit protein eS21-A,YS25
14	b	40S ribosomal protein S22-A	polymer	130	14650.1	1	UniProt (P0C0W1) Pfam (PF00410) In PDB	Saccharomyces cerevisiae (baker's yeast)	RP50,S24,Small ribosomal subunit protein uS8-A,YP58,YS22
15	c	40S ribosomal protein S23-A	polymer	145	16073.9	1	UniProt (P0CX29) Pfam (PF00164) In PDB	Saccharomyces cerevisiae (baker's yeast)	RP37,S28,Small ribosomal subunit protein uS12-A,YS14
16	d	40S ribosomal protein S24-A	polymer	135	15362.8	1	UniProt (P0CX31) Pfam (PF01282) In PDB	Saccharomyces cerevisiae (baker's yeast)	RP50,Small ribosomal subunit protein eS24-A
17	f	40S ribosomal protein S27-A	polymer	82	8893.4	1	UniProt (P35997) Pfam (PF01667) In PDB	Saccharomyces cerevisiae (baker's yeast)	RP61,Small ribosomal subunit protein eS27-A,YS20
18	g	40S ribosomal protein S30-A	polymer	63	7137.5	1	UniProt (P0CX33) Pfam (PF04758) In PDB	Saccharomyces cerevisiae (baker's yeast)	Small ribosomal subunit protein eS30-A

Sequence modifications

x: 1 - 307 (UniProt: P38747)

PDB	External Database	Details
Ser 178	Cys 178	engineered mutation

y: 1 - 76 (UniProt: P05759)

PDB	External Database	Details
Pro 19	Ser 19	conflict
Glu 24	Asp 24	conflict
Ala 28	Ser 28	conflict

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	910077.2
All*	Total formula weight	910077.2

*Water molecules are not included.