8C4Z
ProteinT protease, inactive mutant S224A
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Extracellular serine proteinase | polymer | 112 | 12053.7 | 1 | UniProt (P80146) In PDB | Thermus sp. Rt41A | |
2 | B | Extracellular serine proteinase | polymer | 282 | 29127.4 | 1 | UniProt (P80146) In PDB | Thermus sp. Rt41A | |
3 | B | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
4 | B | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
5 | B | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
6 | water | water | 18.0 | 463 | Chemie (HOH) |
Sequence modifications
B: 1 - 278 (UniProt: P80146)
PDB | External Database | Details |
---|---|---|
Pro -3 | - | expression tag |
Ala -2 | - | expression tag |
Met -1 | - | expression tag |
Ala 0 | - | expression tag |
Ala 224 | Ser 356 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 41181.1 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 156.5 | |
All* | Total formula weight | 41337.6 |