8C4Z
ProteinT protease, inactive mutant S224A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Extracellular serine proteinase | polymer | 112 | 12053.7 | 1 | UniProt (P80146) Pfam (PF05922) | Thermus sp. Rt41A | |
| 2 | B (B) | Extracellular serine proteinase | polymer | 282 | 29127.4 | 1 | UniProt (P80146) Pfam (PF00082) | Thermus sp. Rt41A | |
| 3 | C (B) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 4 | D (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | E (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | F, G (A, B) | water | water | 18.0 | 463 | Chemie (HOH) |
Sequence modifications
B: 1 - 278 (UniProt: P80146)
| PDB | External Database | Details |
|---|---|---|
| Pro -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Ala 224 | Ser 356 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 41181.1 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 156.5 | |
| All* | Total formula weight | 41337.6 |
