8C4X

PAM Protease

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Protease	polymer	276	27720.3	1	UniProt (A0A0U5AS91) Pfam (PF00082) In PDB	Actinomadura keratinilytica
2	B	Protease	polymer	81	8389.6	1	UniProt (A0A0U5AS91) Pfam (PF05922) In PDB	Actinomadura keratinilytica
3	A	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
4	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	2	Chemie (TRS)
5	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
6		water	water		18.0	396	Chemie (HOH)

Sequence modifications

A: 1 - 276 (UniProt: A0A0U5AS91)

PDB	External Database	Details
Asn 4	Thr 114	conflict
Leu 11	Ile 121	conflict
Arg 23	Asn 133	conflict
Ser 48	Pro 158	conflict
Asp 49	Asn 159	conflict
Gly 81	Ser 191	conflict
Ala 82	Thr 192	conflict
Ala 83	Ser 193	conflict
Ala 89	Ser 199	conflict
Ser 118	Gly 228	conflict
Leu 138	Ile 248	conflict
Ser 147	Asn 257	conflict
Ser 148	Ala 258	conflict
Val 154	Ala 264	conflict
Thr 160	Asn 270	conflict
Ala 208	Thr 318	conflict
Thr 230	Val 340	conflict
Val 251	Thr 361	conflict
Val 263	Pro 373	conflict
Leu 269	Arg 379	conflict

B: 12 - 81 (UniProt: A0A0U5AS91)

PDB	External Database	Details
Ala 1	-	expression tag
Pro 2	-	expression tag
Ala 3	-	expression tag
Val 4	-	expression tag
Pro 5	-	expression tag
Val 6	-	expression tag
Ala 7	-	expression tag
Met 8	-	expression tag
Ala 9	-	expression tag
Ala 10	-	expression tag
Ala 11	-	expression tag
Ala 16	Thr 45	conflict
Ala 34	Ser 63	conflict
His 42	Tyr 71	conflict
Asp 58	Ala 87	conflict
Ser 61	Ala 90	conflict
Thr 79	Lys 108	conflict
Val 80	Ala 109	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	36109.9
Non-Polymers*	Number of molecules	4
	Total formula weight	402.5
All*	Total formula weight	36512.4

*Water molecules are not included.