8C4V

Hantaan virus polymerase in replication elongation state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	RNA-directed RNA polymerase L	polymer	2173	249432.5	1	UniProt (P23456) Pfam (PF21991) Pfam (PF12426) Pfam (PF04196) In PDB	Hantaan virus 76-118	Protein L,Large structural protein,RdRp,Replicase,Transcriptase
2	H	RNA (5'-R(P*AP*GP*GP*AP*GP*UP*AP*UP*CP*CP*AP*CP*CP*GP*CP*AP*AP*GP*A)-3')	polymer	20	6429.9	1		Hantaan virus 76-118
3	V, T, S	RNA (5'-R(P*AP*CP*UP*AP*CP*UP*A)-3')	polymer	25	7851.6	3		Hantaan virus 76-118
4	P	RNA (5'-R(P*AP*GP*UP*AP*GP*UP*AP*GP*A)-3')	polymer	25	8092.9	1		Hantaan virus 76-118
5	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 1 - 2151 (UniProt: P23456)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Asp -13	-	expression tag
Tyr -12	-	expression tag
Asp -11	-	expression tag
Ile -10	-	expression tag
Pro -9	-	expression tag
Thr -8	-	expression tag
Thr -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ala 97	Asp 97	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	287510.1
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	287534.5

*Water molecules are not included.